| RRC ID |
3142
|
| 著者 |
Hanajima-Ozawa M, Matsuzawa T, Fukui A, Kamitani S, Ohnishi H, Abe A, Horiguchi Y, Miyake M.
|
| タイトル |
Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals.
|
| ジャーナル |
Infect Immun
|
| Abstract |
Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes induce localized actin polymerization at the cytoplasmic face of the plasma membrane or within the host cytoplasm, creating unique actin-rich structures termed pedestals or actin tails. The process is known to be mediated by the actin-related protein 2 and 3 (Arp2/3) complex, which in these cases acts downstream of neural Wiskott-Aldrich syndrome protein (N-WASP) or of a listerial functional homolog of WASP family proteins. Here, we show that zonula occludens-1 (ZO-1), a protein in the tight junctions of polarized epithelial cells, is recruited to actin tails and pedestals. Immunocytochemical analysis revealed that ZO-1 was stained most in the distal part of the actin-rich structures, and the incorporation was mediated by the proline-rich region of the ZO-1 molecule. The direct clustering of membrane-targeted Nck, which is known to activate the N-WASP-Arp2/3 pathway, triggered the formation of the ZO-1-associated actin tails. The results suggest that the activation of the Arp2/3 complex downstream of N-WASP or a WASP-related molecule is a key to the formation of the particular actin-rich structures that bind with ZO-1. We propose that an analysis of the recruitment on a molecular basis will lead to an understanding of how ZO-1 recognizes a distinctive actin-rich structure under pathophysiological conditions.
|
| 巻・号 |
75(2)
|
| ページ |
565-73
|
| 公開日 |
2007-2-1
|
| DOI |
10.1128/IAI.01479-06
|
| PII |
IAI.01479-06
|
| PMID |
17118974
|
| PMC |
PMC1828484
|
| MeSH |
Actin-Related Protein 2 / metabolism
Actin-Related Protein 3 / metabolism
Actins / metabolism*
Adaptor Proteins, Signal Transducing
Animals
Cytoplasm / chemistry
Epithelial Cells / microbiology*
Escherichia coli / physiology*
HeLa Cells
Humans
Immunohistochemistry
Listeria monocytogenes / physiology*
Membrane Proteins / metabolism*
Mice
Microscopy, Fluorescence
NIH 3T3 Cells
Oncogene Proteins / physiology
Phosphoproteins / metabolism*
Shigella flexneri / physiology*
Zonula Occludens-1 Protein
|
| IF |
3.201
|
| 引用数 |
26
|
|
WOS 分野
|
INFECTIOUS DISEASES
IMMUNOLOGY
|
| リソース情報 |
| 病原微生物 |
L. monocytogenes(RIMD 1205001)
S. flexneri 2a? |
