論文 - 詳細
| RRC ID | 3142 |
|---|---|
| 著者 | Hanajima-Ozawa M, Matsuzawa T, Fukui A, Kamitani S, Ohnishi H, Abe A, Horiguchi Y, Miyake M. |
| タイトル | Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes recruit a junctional protein, zonula occludens-1, to actin tails and pedestals. |
| ジャーナル | Infect Immun |
| Abstract |
Enteropathogenic Escherichia coli, Shigella flexneri, and Listeria monocytogenes induce localized actin polymerization at the cytoplasmic face of the plasma membrane or within the host cytoplasm, creating unique actin-rich structures termed pedestals or actin tails. The process is known to be mediated by the actin-related protein 2 and 3 (Arp2/3) complex, which in these cases acts downstream of neural Wiskott-Aldrich syndrome protein (N-WASP) or of a listerial functional homolog of WASP family proteins. Here, we show that zonula occludens-1 (ZO-1), a protein in the tight junctions of polarized epithelial cells, is recruited to actin tails and pedestals. Immunocytochemical analysis revealed that ZO-1 was stained most in the distal part of the actin-rich structures, and the incorporation was mediated by the proline-rich region of the ZO-1 molecule. The direct clustering of membrane-targeted Nck, which is known to activate the N-WASP-Arp2/3 pathway, triggered the formation of the ZO-1-associated actin tails. The results suggest that the activation of the Arp2/3 complex downstream of N-WASP or a WASP-related molecule is a key to the formation of the particular actin-rich structures that bind with ZO-1. We propose that an analysis of the recruitment on a molecular basis will lead to an understanding of how ZO-1 recognizes a distinctive actin-rich structure under pathophysiological conditions. |
| 巻・号 | 75(2) |
| ページ | 565-73 |
| 公開日 | 2007-2-1 |
| DOI | 10.1128/IAI.01479-06 |
| PII | IAI.01479-06 |
| PMID | 17118974 |
| PMC | PMC1828484 |
| MeSH | Actin-Related Protein 2 / metabolism Actin-Related Protein 3 / metabolism Actins / metabolism* Adaptor Proteins, Signal Transducing Animals Cytoplasm / chemistry Epithelial Cells / microbiology* Escherichia coli / physiology* HeLa Cells Humans Immunohistochemistry Listeria monocytogenes / physiology* Membrane Proteins / metabolism* Mice Microscopy, Fluorescence NIH 3T3 Cells Oncogene Proteins / physiology Phosphoproteins / metabolism* Shigella flexneri / physiology* Zonula Occludens-1 Protein |
| IF | 3.201 |
| 引用数 | 26 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 病原微生物 | L. monocytogenes(RIMD 1205001) S. flexneri 2a? |