RRC ID 3149
著者 Pandey K, Yanagi T, Pandey BD, Mallik AK, Sherchand JB, Kanbara H.
タイトル Characterization of Leishmania isolates from Nepalese patients with visceral leishmaniasis.
ジャーナル Parasitol Res
Abstract In Nepal, visceral leishmaniasis (VL) is endemic in 13 districts of the central and eastern regions. A total of 166 bone-marrow aspirates were obtained from patients with suspected VL. Ninety-seven were identified as positive by microscopy, and 29 of those were successfully isolated and cultured. We characterized these isolates by molecular analysis and by their ability to infect mice. PCR-restriction fragment length polymorphism analysis of the mini-exon and the cysteine proteinase b gene showed that all isolates were Leishmania donovani, and the restriction pattern of the Nepalese isolates corresponded to the standard Indian strain of L. donovani but differed from that of the Kenyan strain. The single-strand conformation polymorphism analysis of ribosomal internal transcribed spacer showed no genetic heterogeneity within Nepalese isolates. Intraperitoneal inoculation with the promastigotes of all isolates resulted in amastigote proliferation in the spleen of 20 nude mice, of which ten isolates were highly infective, and ten were moderately infective, including one BALB/c mouse. Of the 20 amastigotes isolated from the spleen of nude mice, only the ten highly infective isolates infected BALB/c mice, of which, two isolates were considered to have low infectivity, three isolates were considered to be moderately infective, and five isolates were considered to be highly infective.
巻・号 100(6)
ページ 1361-9
公開日 2007-5-1
DOI 10.1007/s00436-007-0464-4
PMID 17310397
MeSH Adult Aged Animals Child Child, Preschool Cricetinae DNA, Protozoan / genetics DNA, Ribosomal Spacer / genetics Female Humans Leishmania / classification* Leishmania / genetics* Leishmania / isolation & purification Leishmaniasis, Visceral / epidemiology Leishmaniasis, Visceral / parasitology* Male Mesocricetus Mice Mice, Inbred BALB C Mice, Nude Middle Aged Nepal / epidemiology
IF 1.641
引用数 18
WOS 分野 PARASITOLOGY
リソース情報
病原微生物 L. donovani(D5 D7 D10 D14 D19 D21 D26 D29 D34 D36 D38 D42 D45 D67? D70? D71? D77? D78? D98? D99? T4? T6 T9 T11 T13? T14? T22? T25? T27?)