RRC ID 3153
著者 Masue N, Deguchi T, Yokoi S, Yamada T, Ohkusu K, Ezaki T.
タイトル System for simultaneous detection of 16 pathogens related to urethritis to diagnose mixed infection.
ジャーナル Int J Urol
Abstract OBJECTIVE:Urethritis is not always caused by a single pathogen, and isolation of more than two pathogens from one patient is not uncommon. We developed a method to simultaneously detect 16 pathogens related to urethritis.
METHODS:We designed specific primers used for amplification of urethritis pathogens in our 16-well microplate assay. Sixteen microliters of each reaction mixture containing template DNA was added to each well to amplify 16 pathogens simultaneously.
RESULTS:After we evaluated the specificity and sensitivity of this microplate polymerase chain reaction method, we used it to detect pathogens in clinical samples. Of 163 clinical samples, 49.7% (81/163) were positive for specific pathogens, and 6.7% (11/163) showed mixed infection. A specific pathogen was not identified in 43.6% (71/163) of cases.
CONCLUSIONS:We developed a 16-well microplate assay with 16 specific primers to identify pathogens associated with urethritis.
巻・号 14(1)
ページ 39-42
公開日 2007-1-1
DOI 10.1111/j.1442-2042.2006.01660.x
PII IJU1660
PMID 17199858
MeSH Humans Infections / complications Infections / microbiology Microbiological Techniques / methods Polymerase Chain Reaction Urethritis / microbiology*
IF 2.445
引用数 6
WOS 分野 UROLOGY & NEPHROLOGY
リソース情報
病原微生物