RRC ID 316
Author Ejima A, Tsuda M, Takeo S, Ishii K, Matsuo T, Aigaki T.
Title Expression level of sarah, a homolog of DSCR1, is critical for ovulation and female courtship behavior in Drosophila melanogaster.
Journal Genetics
Abstract To better understand the genetic bases of postmating responses in Drosophila melanogaster females, we screened a collection of P{GS} insertion lines and identified two insertions in sarah (sra), whose misexpression in the nervous system induced high levels of ovulation in virgins. The gene sra encodes a protein similar to human Down syndrome critical region 1 (DSCR1). The ovulation phenotype was reproduced in transgenic virgins expressing UAS-sra in the nervous system. The flies also extruded the ovipositor toward courting males as seen in wild-type mated females, supporting the notion that ovulation and behavioral patterns are physiologically coupled. The sra insertions were found to be hypomorphic alleles with reduced expression levels. Females homozygous for these alleles show: (1) spontaneous ovulation in virgins, (2) sterility with impaired meiotic progression, and (3) compromised postmating responses with lower ovulation level, higher remating rate, and shorter period for restoration of receptivity. No obvious defects were observed in the homozygous males. The gene sra is predominantly expressed in oocytes, nurse cells, and the nervous system. Taken together, these results indicate that the expression level of sra is critical for ovulation and female courtship behavior, including their postmating changes.
Volume 168(4)
Pages 2077-87
Published 2004-12
DOI 10.1534/genetics.104.029934
PII 168/4/2077
PMID 15611177
PMC PMC1448750
MeSH Amino Acid Sequence Animals Brain / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Female Genetic Vectors Intracellular Signaling Peptides and Proteins Molecular Sequence Data Muscle Proteins / genetics* Muscle Proteins / physiology Oogenesis / genetics Oogenesis / physiology Ovulation / physiology* Sexual Behavior, Animal / physiology*
IF 3.564
Times Cited 14