| RRC ID |
31697
|
| 著者 |
Sakamaki K, Tsukumo S, Yonehara S.
|
| タイトル |
Molecular cloning and characterization of mouse caspase-8.
|
| ジャーナル |
Eur J Biochem
|
| Abstract |
Fas (APO-1/CD95) is a transmembrane receptor protein which induces apoptosis upon activation. In apoptosis triggered by Fas, a subset of cysteine proteases designated caspases is activated, playing a central role as effector molecules. Among these caspases, human caspase-8 (FLICE/MACH/Mch5) has been isolated and shown to be indispensable for Fas-mediated apoptotic signaling. In this study, we isolated the mouse homologue to human caspase-8 from a BaF3 cell cDNA library. This molecule conserved the death effector domain (DED) and protease domain as detected in human caspase-8, and was capable of inducing apoptosis in KB and Rat-1 cells when overexpressed. Expression of caspase-8 was detected in the various tissues of adult mouse and in embryos at 9.5 days and 17.5 days of development by Northern-blot analysis. Further, we isolated a chromosomal gene for caspase-8 from a mouse genomic library and analyzed the genomic structure of the isolated gene. This gene consisted of eight exons and seven introns spanning about 26 kb in the coding region.
|
| 巻・号 |
253(2)
|
| ページ |
399-405
|
| 公開日 |
1998-4-15
|
| DOI |
10.1046/j.1432-1327.1998.2530399.x
|
| PMID |
9654089
|
| MeSH |
Amino Acid Sequence
Animals
Apoptosis
Base Sequence
Caenorhabditis elegans Proteins
Caspase 8
Caspase 9
Caspases*
Cells, Cultured
Cysteine Endopeptidases / genetics*
Cysteine Endopeptidases / isolation & purification
Cysteine Endopeptidases / metabolism
Humans
Mice
Molecular Sequence Data
RNA, Messenger / metabolism
Sequence Alignment
Sequence Homology, Amino Acid
|
| 引用数 |
43
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pmmCasp8 (Mouse caspase-8) (RDB08071) |
