RRC ID 31697
Author Sakamaki K, Tsukumo S, Yonehara S.
Title Molecular cloning and characterization of mouse caspase-8.
Journal Eur. J. Biochem.
Abstract Fas (APO-1/CD95) is a transmembrane receptor protein which induces apoptosis upon activation. In apoptosis triggered by Fas, a subset of cysteine proteases designated caspases is activated, playing a central role as effector molecules. Among these caspases, human caspase-8 (FLICE/MACH/Mch5) has been isolated and shown to be indispensable for Fas-mediated apoptotic signaling. In this study, we isolated the mouse homologue to human caspase-8 from a BaF3 cell cDNA library. This molecule conserved the death effector domain (DED) and protease domain as detected in human caspase-8, and was capable of inducing apoptosis in KB and Rat-1 cells when overexpressed. Expression of caspase-8 was detected in the various tissues of adult mouse and in embryos at 9.5 days and 17.5 days of development by Northern-blot analysis. Further, we isolated a chromosomal gene for caspase-8 from a mouse genomic library and analyzed the genomic structure of the isolated gene. This gene consisted of eight exons and seven introns spanning about 26 kb in the coding region.
Volume 253(2)
Pages 399-405
Published 1998-4-15
PMID 9654089
MeSH Amino Acid Sequence Animals Apoptosis Base Sequence Caenorhabditis elegans Proteins Caspase 8 Caspase 9 Caspases* Cells, Cultured Cysteine Endopeptidases / genetics* Cysteine Endopeptidases / isolation & purification Cysteine Endopeptidases / metabolism Humans Mice Molecular Sequence Data RNA, Messenger / metabolism Sequence Alignment Sequence Homology, Amino Acid
Times Cited 39
DNA material pmmCasp8 (Mouse caspase-8) (RDB08071)