論文 - 詳細
| RRC ID | 31697 |
|---|---|
| 著者 | Sakamaki K, Tsukumo S, Yonehara S. |
| タイトル | Molecular cloning and characterization of mouse caspase-8. |
| ジャーナル | Eur J Biochem |
| Abstract |
Fas (APO-1/CD95) is a transmembrane receptor protein which induces apoptosis upon activation. In apoptosis triggered by Fas, a subset of cysteine proteases designated caspases is activated, playing a central role as effector molecules. Among these caspases, human caspase-8 (FLICE/MACH/Mch5) has been isolated and shown to be indispensable for Fas-mediated apoptotic signaling. In this study, we isolated the mouse homologue to human caspase-8 from a BaF3 cell cDNA library. This molecule conserved the death effector domain (DED) and protease domain as detected in human caspase-8, and was capable of inducing apoptosis in KB and Rat-1 cells when overexpressed. Expression of caspase-8 was detected in the various tissues of adult mouse and in embryos at 9.5 days and 17.5 days of development by Northern-blot analysis. Further, we isolated a chromosomal gene for caspase-8 from a mouse genomic library and analyzed the genomic structure of the isolated gene. This gene consisted of eight exons and seven introns spanning about 26 kb in the coding region. |
| 巻・号 | 253(2) |
| ページ | 399-405 |
| 公開日 | 1998-4-15 |
| DOI | 10.1046/j.1432-1327.1998.2530399.x |
| PMID | 9654089 |
| MeSH | Amino Acid Sequence Animals Apoptosis Base Sequence Caenorhabditis elegans Proteins Caspase 8 Caspase 9 Caspases* Cells, Cultured Cysteine Endopeptidases / genetics* Cysteine Endopeptidases / isolation & purification Cysteine Endopeptidases / metabolism Humans Mice Molecular Sequence Data RNA, Messenger / metabolism Sequence Alignment Sequence Homology, Amino Acid |
| 引用数 | 43 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pmmCasp8 (Mouse caspase-8) (RDB08071) |