論文 - 詳細
| RRC ID | 31700 |
|---|---|
| 著者 | Kawaguchi A, Utsumi N, Morita M, Ohya A, Wada S. |
| タイトル | Application of the cis-regulatory region of a heat-shock protein 70 gene to heat-inducible gene expression in the ascidian Ciona intestinalis. |
| ジャーナル | Genesis |
| Abstract |
Temporally controlled induction of gene expression is a useful technique for analyzing gene function. To make such a technique possible in Ciona intestinalis embryos, we employed the cis-regulatory region of the heat-shock protein 70 (HSP70) gene Ci-HSPA1/6/7-like for heat-inducible gene expression in C. intestinalis embryos. We showed that Ci-HSPA1/6/7-like becomes heat shock-inducible by the 32-cell stage during embryogenesis. The 5'-upstream region of Ci-HSPA1/6/7-like, which contains heat-shock elements indispensable for heat-inducible gene expression, induces the heat shock-dependent expression of a reporter gene in the whole embryo from the 32-cell to the middle gastrula stages and in progressively restricted areas of embryos in subsequent stages. We assessed the effects of heat-shock treatments in different conditions on the normality of embryos and induction of transgene expression. We evaluated the usefulness of this technique through overexpression experiments on the well-characterized, developmentally relevant gene, Ci-Bra, and showed that this technique is applicable for inferring the gene function in C. intestinalis. |
| 巻・号 | 53(1) |
| ページ | 170-82 |
| 公開日 | 2015-1-1 |
| DOI | 10.1002/dvg.22834 |
| PMID | 25366274 |
| MeSH | Animals Ciona intestinalis / embryology* Ciona intestinalis / genetics* Gene Expression Regulation, Developmental* Genes, Reporter HSP70 Heat-Shock Proteins / genetics* Heat-Shock Response Hot Temperature Regulatory Sequences, Nucleic Acid* |
| IF | 1.76 |
| 引用数 | 2 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |