RRC ID 31702
Author Kioka N, Tsubota J, Kakehi Y, Komano T, Gottesman MM, Pastan I, Ueda K.
Title P-glycoprotein gene (MDR1) cDNA from human adrenal: normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistance.
Journal Biochem. Biophys. Res. Commun.
Abstract We isolated a full-length MDR1 cDNA from human adrenal where P-glycoprotein is expressed at high level. The deduced amino acid sequence shows two amino acid differences from the sequence of P-glycoprotein obtained from colchicine-selected multidrug resistant cultured cells. The amino acid substitution Gly----Val at codon 185 in P-glycoprotein from colchicine resistant cells occurred during selection of cells in colchicine. As previously reported, cells transfected with the MDR1 cDNA carrying Val185 acquire increased resistance to colchicine compared to other drugs. The other amino acid substitution Ser----Ala at codon 893 probably reflects genetic polymorphism. The MDR1 gene, the major member of the P-glycoprotein gene family expressed in human adrenal, is sufficient to confer multidrug-resistance on culture cells.
Volume 162(1)
Pages 224-31
Published 1989-7-14
PII 0006-291X(89)91985-2
PMID 2568832
MeSH ATP-Binding Cassette, Sub-Family B, Member 1 Adrenal Glands / analysis* Adrenal Glands / physiology Alanine / genetics Amino Acid Sequence Base Sequence Cell Line Cloning, Molecular DNA / isolation & purification* Drug Resistance Genetic Vectors Glycine / genetics* Humans Membrane Glycoproteins / genetics* Membrane Glycoproteins / physiology Molecular Sequence Data Polymorphism, Genetic* Serine / genetics Tumor Cells, Cultured / analysis Tumor Cells, Cultured / metabolism
IF 2.559
Times Cited 157
DNA material Human MDR1 cDNA (RDB01372)