RRC ID 31703
著者 Chen CJ, Chin JE, Ueda K, Clark DP, Pastan I, Gottesman MM, Roninson IB.
タイトル Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cells.
ジャーナル Cell
Abstract Resistance of tumor cells to multiple cytotoxic drugs is a major impediment to cancer chemotherapy. Multidrug resistance in human cells is determined by the mdr1 gene, encoding a high molecular weight membrane glycoprotein (P-glycoprotein). Complete primary structure of human P-glycoprotein has been determined from the cDNA sequence. The protein, 1280 amino acids long, consists of two homologous parts of approximately equal length. Each half of the protein includes a hydrophobic region with six predicted transmembrane segments and a hydrophilic region. The hydrophilic regions share homology with peripheral membrane components of bacterial active transport systems and include potential nucleotide-binding sites. These results are consistent with a function for P-glycoprotein as an energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
巻・号 47(3)
ページ 381-9
公開日 1986-11-7
DOI 10.1016/0092-8674(86)90595-7
PII 0092-8674(86)90595-7
PMID 2876781
MeSH ATP Binding Cassette Transporter, Subfamily B, Member 1 Amino Acid Sequence Bacterial Proteins / analysis Bacterial Proteins / genetics* Base Sequence Biological Transport, Active Cell Line DNA / analysis Drug Resistance / genetics* Glycoproteins / analysis Glycoproteins / genetics* Humans Molecular Weight Neoplasms / drug therapy Protein Conformation
IF 38.637
引用数 1861
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
遺伝子材料 Human MDR1 cDNA (RDB01372)