RRC ID 31714
Author Burger E, Miyaji M, Sano A, Calich VL, Nishimura K, Lenzi HL.
Title Histopathology of paracoccidioidomycotic infection in athymic and euthymic mice: a sequential study.
Journal Am J Trop Med Hyg
Abstract We report the comparative and sequential histologic analysis of lesions developed by Paracoccidioides brasiliensis-infected athymic and euthymic BALB/c mice, using conventional and specific stain to characterize the morphology of fungi and inflammatory cells as well as the extracellular matrix patterns. In both groups, neutrophils and macrophages were the predominant cells; macrophage transition to giant and epithelioid cells was observed. Degenerated fungi and fungal dust were associated with local neutropil infiltration and the presence of pseudoxantomatous macrophages. Paracoccidioides brasiliensis are always found within an extracellular matrix microenvironment. The main differences between lesions developed by nu/+ and nude (nu/nu) mice resided in the more pronounced encapsulating tendency in the former ones. In both groups of animals, lesions in several organs were associated with extensive hemopoiesis. At the first week of infection, there was evidence of fungal destruction and control of its proliferation in both mouse groups. At the fourth and seventh weeks, nu/+ mice maintained the control of the infection, as shown by the scarce numbers of budding yeast cells, while experimental nu/nu mice showed a tumor-like progression of the disease with a striking increase in fungal load in many organs (e.g., omental and mesenteric milky spots, lymph nodes, spleen), some of which present their structure replaced by granulomatous lesions.
Volume 55(2)
Pages 235-42
Published 1996-8-1
DOI 10.4269/ajtmh.1996.55.235
PMID 8780467
MeSH Animals Brain / pathology Child, Preschool Extracellular Matrix / pathology Female Humans Liver / microbiology Liver / pathology Lymph Nodes / pathology Macrophages Male Mesentery / pathology Mice Mice, Inbred BALB C Mice, Nude Neutrophils Omentum / pathology Paracoccidioidomycosis / immunology Paracoccidioidomycosis / pathology* Specific Pathogen-Free Organisms Spleen / pathology
IF 2.126
Times Cited 18
Pathogenic microorganisms IFM 41621 IFM 1629 IFM 46466