RRC ID 31826
Author Mitani-Ueno N, Ogai H, Yamaji N, Ma JF.
Title Physiological and molecular characterization of Si uptake in wild rice species.
Journal Physiol Plant
Abstract Cultivated rice (Oryza sativa) accumulates high concentration of silicon (Si), which is required for its high and sustainable production. High Si accumulation in cultivated rice is achieved by a high expression of both influx (Lsi1) and efflux (Lsi2) Si transporters in roots. Herein, we physiologically investigated Si uptake, isolated and functionally characterized Si transporters in six wild rice species with different genome types. Si uptake by the roots was lower in Oryza rufipogon, Oryza barthii (AA genome), Oryza australiensis (EE genome) and Oryza punctata (BB genome), but similar in Oryza glumaepatula and Oryza meridionalis (AA genome) compared with the cultivated rice (cv. Nipponbare). However, all wild rice species and the cultivated rice showed similar concentration of Si in the shoots when grown in a field. All species with AA genome showed the same amino acid sequence of both Lsi1 and Lsi2 as O. sativa, whereas species with EE and BB genome showed several nucleotide differences in both Lsi1 and Lsi2. However, proteins encoded by these genes also showed transport activity for Si in Xenopus oocyte. The mRNA expression of Lsi1 in all wild rice species was lower than that in the cultivated rice, whereas the expression of Lsi2 was lower in O. rufipogon and O. barthii but similar in other species. Similar cellular localization of Lsi1 and Lsi2 was observed in all wild rice as the cultivated rice. These results indicate that superior Si uptake, the important trait for rice growth, is basically conserved in wild and cultivated rice species.
Volume 151(3)
Pages 200-7
Published 2014-7-1
DOI 10.1111/ppl.12125
PMID 24320720
MeSH Amino Acid Sequence Base Sequence Biological Transport, Active Gene Expression Regulation, Plant Genome, Plant / genetics Genotype Immunohistochemistry Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism Microscopy, Confocal Molecular Sequence Data Oryza / genetics Oryza / metabolism* Plant Proteins / genetics Plant Proteins / metabolism Plant Roots / genetics Plant Roots / metabolism Poaceae / classification Poaceae / genetics Poaceae / metabolism* Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Silicon / metabolism* Species Specificity
IF 3.0
Times Cited 10
Rice W0630 W0720 W1169 W1627 W0008 W1514