論文 - 詳細
| RRC ID | 31871 |
|---|---|
| 著者 | Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K, Yoshikai Y. |
| タイトル | A genome-wide analysis identifies a notch-RBP-Jκ-IL-7Rα axis that controls IL-17-producing γδ T cell homeostasis in mice. |
| ジャーナル | J Immunol |
| Abstract |
Notch signaling is an important regulator for the development and function of both αβ and γδ T cells, whereas roles of Notch signaling in T cell maintenance remain unclear. We reported previously that the Notch-Hes1 pathway was involved in the intrathymic development of naturally occurring IL-17-producing (IL-17(+)) γδ T cells. To gain insight into additional roles for the Notch axis in the homeostasis of γδ T cells, we performed a genome-wide analysis of Notch target genes and identified the novel promoter site of IL-7Rα driven by the Notch-RBP-Jκ pathway. Constitutive Notch signaling had the potential to induce IL-7Rα expression on γδ T cells in vivo, as well as in vitro, whereas conditional deletion of RBP-Jκ abrogated IL-7Rα expression, but not Hes1 expression, by γδ T cells and selectively reduced the pool size of IL-7Rα(high) IL-17(+) γδ T cells in the periphery. In the absence of IL-7Rα-mediated signaling, IL-17(+) γδ T cells were barely maintained in adult mice. Addition of exogenous IL-7 in vitro selectively expanded IL-17(+) γδ T cells. Thus, our results revealed a novel role for the Notch-RBP-Jκ-IL-7Rα axis that is independent of Hes1 for homeostasis of IL-17(+) γδ T cells. |
| 巻・号 | 194(1) |
| ページ | 243-51 |
| 公開日 | 2015-1-1 |
| DOI | 10.4049/jimmunol.1401619 |
| PII | jimmunol.1401619 |
| PMID | 25429074 |
| MeSH | Animals Antibodies / immunology Basic Helix-Loop-Helix Transcription Factors / biosynthesis Cell Proliferation / drug effects Genome-Wide Association Study Homeodomain Proteins / biosynthesis Homeostasis Immunoglobulin J Recombination Signal Sequence-Binding Protein / genetics Immunoglobulin J Recombination Signal Sequence-Binding Protein / immunology* Interferon-gamma / biosynthesis Interleukin-17 / biosynthesis* Interleukin-7 / biosynthesis Interleukin-7 / pharmacology Mice Mice, Inbred C57BL Mice, Knockout Receptor, Notch1 / immunology* Receptors, Antigen, T-Cell, alpha-beta / immunology Receptors, Antigen, T-Cell, gamma-delta / immunology* Receptors, Interleukin-7 / biosynthesis Receptors, Interleukin-7 / immunology* Signal Transduction T-Lymphocytes / immunology Transcription Factor HES-1 |
| IF | 4.886 |
| 引用数 | 13 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01071 |