Reference - Detail
| RRC ID | 31881 |
|---|---|
| Author | Tone Y, Kawahara M, Kawaguchi D, Ueda H, Nagamune T. |
| Title | Death signalobody: inducing conditional cell death in response to a specific antigen. |
| Journal | Hum Gene Ther Methods |
| Abstract |
As the possibility of tumorigenesis and undesirable immune responses in patients cannot be completely excluded in gene and cell therapies, a conditional death switch to eliminate the therapeutic cells would be a valuable tool to enhance the safety of these therapies. A few ligand-receptor conditional death switches have already been developed; however, they cannot be used if patients exhibit side effects upon administration of the ligand. Here we demonstrate a death-inducing chimeric antibody named "death signalobody," in which the antigen-antibody system, having virtually infinite ligand-receptor combinations, is utilized for the activation of death signaling. We designed a death signalobody named "SFas," which has an antifluorescein single-chain variable fragment and the cytoplasmic domain of Fas. SFas efficiently induced conditional apoptosis in murine pro-B Ba/F3 cells in response to fluorescein-conjugated bovine serum albumin. Moreover, SFas was also able to induce antigen-dependent conditional apoptosis in human cancer cell lines. The death signalobody technique will be a valuable tool for the conditional elimination of cells of interest in multiple therapeutic applications. |
| Volume | 24(3) |
| Pages | 141-50 |
| Published | 2013-6-1 |
| DOI | 10.1089/hgtb.2012.147 |
| PMID | 23470213 |
| MeSH | Animals Antigen-Antibody Complex Antigens / immunology Antigens / metabolism Apoptosis* Cell Line Cell Survival Fluorescein / chemistry Fluorescein / metabolism Genetic Vectors / genetics Genetic Vectors / metabolism Humans Mice Recombinant Fusion Proteins / genetics* Recombinant Fusion Proteins / metabolism Retroviridae / genetics Serum Albumin, Bovine / chemistry Serum Albumin, Bovine / metabolism Single-Chain Antibodies / genetics* Single-Chain Antibodies / metabolism Transduction, Genetic fas Receptor / genetics* fas Receptor / metabolism |
| IF | 2.446 |
| Times Cited | 7 |
| WOS Category | MEDICINE, RESEARCH & EXPERIMENTAL BIOTECHNOLOGY & APPLIED MICROBIOLOGY GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pRx hFAS-i-hCD25 (RDB01702) |