RRC ID 3190
著者 Kawakami H, Iwura T, Takata M, Sekimizu K, Hiraga S, Katayama T.
タイトル Arrest of cell division and nucleoid partition by genetic alterations in the sliding clamp of the replicase and in DnaA.
ジャーナル Mol Genet Genomics
Abstract In Escherichia coli, an interaction between the replication initiator DnaA and the sliding clamp protein, the beta subunit (DnaN) of DNA polymerase III, is required to regulate the chromosomal replication cycle. We report here that colony formation by, and cell division of, the temperature (42 degrees C)-sensitive dnaN59 mutant are inhibited at 34-35 degrees C when DnaA is moderately (4-to 8-fold ) overexpressed, although chromosomal replication and the beta subunit-dependent regulation of DnaA activity are not significantly inhibited. Immunoblotting analysis revealed that the beta subunit is abundant (present at a level of about 5000 dimers per cell) at 34 degrees C, and its concentration per unit cell volume was practically unaffected in the dnaN59 mutant by the overexpression of DnaA. The dnaN mutant cells that overexpress DnaA become filamentous at 34 degrees C via an sfiA-independent pathway, different from that activated by the SOS response. This filamentation is accompanied by inhibition of nucleoid partition and FtsZ ring formation. In the dnaN59 mutant, oversupply of DnaA may disturb the coordinated action of cell cycle-regulating molecules, thus leading to the inhibition of these events.
巻・号 266(2)
ページ 167-79
公開日 2001-10-1
DOI 10.1007/s004380100546
PMID 11683257
MeSH Bacterial Proteins / genetics* Bacterial Proteins / metabolism Cell Division / genetics* Chromosomes, Bacterial / genetics Cytoskeletal Proteins* DNA Polymerase III / genetics DNA Polymerase III / metabolism DNA Replication / genetics DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism DNA-Directed DNA Polymerase / genetics* DNA-Directed DNA Polymerase / metabolism Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli / metabolism Fluorescent Antibody Technique Gene Expression Regulation, Bacterial Genetic Complementation Test Immunoblotting Mutation Plasmids / genetics Protein Binding Protein Subunits Temperature
IF 2.797
引用数 20
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME7879(KH5402-1)