RRC ID |
31902
|
著者 |
Tamura M, Watanabe K, Mikami Y, Yazawa K, Nishimura K.
|
タイトル |
Molecular characterization of new clinical isolates of Candida albicans and C. dubliniensis in Japan: analysis reveals a new genotype of C. albicans with group I intron.
|
ジャーナル |
J Clin Microbiol
|
Abstract |
The genetic diversity of recent clinical isolates of Candida albicans in Japan was studied on the basis of amplified DNA band lengths determined with a specific PCR primer reported to have been designed to span a transposable intron region in the 25S rRNA gene. Our analyses of 301 clinical isolates of C. albicans showed that they could be classified into five genotypes: genotype A (172 isolates), genotype B (66 isolates), genotype C (56 isolates), genotype D (C. dubliniensis; 5 isolates), and a new genotype (designated genotype E; 2 isolates). The new genotype E was characterized to have a group I intron-like sequence, which is longer than hitherto reported ones and which has a nucleotide sequence length of 962 bp. Our analysis of the 962-bp sequence indicated that it is composed of an intron similar to that of C. dubliniensis of 621 bp with a 341-bp insertion. Analysis of the sequence of the internal transcribed spacer (ITS) region of the genotype E strain showed that its sequence is identical to those of strains of other genotypes, with only a few base substitution differences. Throughout the study, the possible horizontal transfer of the group I intron between C. dubliniensis and C. albicans was suggested. A high degree of correlation between the presence of a group I intron in C. albicans genotype E and susceptibility to the antifungal agent flucytosine was observed. The five isolates of C. dubliniensis examined in the present study showed genetic diversity when they were compared by randomly amplified polymorphic DNA fingerprinting pattern analysis, and this diversity was also confirmed by the analysis of ITS region sequences.
|
巻・号 |
39(12)
|
ページ |
4309-15
|
公開日 |
2001-12-1
|
DOI |
10.1128/JCM.39.12.4309-4315.2001
|
PMID |
11724837
|
PMC |
PMC88541
|
MeSH |
Antifungal Agents / pharmacology
Base Sequence
Candida / classification*
Candida / drug effects
Candida / genetics
Candida / isolation & purification
Candida albicans / classification*
Candida albicans / drug effects
Candida albicans / genetics
Candida albicans / isolation & purification
Candidiasis / epidemiology*
Candidiasis / microbiology
DNA, Fungal / genetics
DNA, Ribosomal Spacer / genetics
Genotype
Humans
Introns / genetics*
Japan / epidemiology
Microbial Sensitivity Tests
Molecular Sequence Data
Mycological Typing Techniques
Phenotype
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
|
IF |
5.897
|
引用数 |
71
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
病原微生物 |
IFM 40213
IFM 40214
IFM 48313
IFM 49826
IFM 49827
IFM 49828
IFM 49829
IFM 49830
IFM 49831
IFM 49832
IFM 49833
IFM 52112 |