| RRC ID |
31910
|
| 著者 |
Nakayama M, Hosoya K, Tomiyama D, Tsugukuni T, Matsuzawa T, Imanishi Y, Yaguchi T.
|
| タイトル |
Method for rapid detection and identification of chaetomium and evaluation of resistance to peracetic acid.
|
| ジャーナル |
J Food Prot
|
| Abstract |
In the beverage industry, peracetic acid has been increasingly used as a disinfectant for the filling machinery and environment due to merits of leaving no residue, it is safe for humans, and its antiseptic effect against fungi and endospores of bacteria. Recently, Chaetomium globosum and Chaetomium funicola were reported resistant to peracetic acid; however, little is known concerning the detail of peracetic acid resistance. Therefore, we assessed the peracetic acid resistance of the species of Chaetomium and related genera under identical conditions and made a thorough observation of the microstructure of their ascospores by transmission electron microscopy. The results of analyses revealed that C. globosum and C. funicola showed the high resistance to peracetic acid (a 1-D antiseptic effect after 900 s and 3-D antiseptic effect after 900 s) and had thick cell walls of ascospores that can impede the action mechanism of peracetic acid. We also developed specific primers to detect the C. globosum clade and identify C. funicola by using PCR to amplify the β-tubulin gene. PCR with the primer sets designed for C. globosum (Chae 4F/4R) and C. funicola (Cfu 2F/2R) amplified PCR products specific for the C. globosum clade and C. funicola, respectively. PCR with these two primer sets did not detect other fungi involved in food spoilage and environmental contamination. This detection and identification method is rapid and simple, with extremely high specificity.
|
| 巻・号 |
76(6)
|
| ページ |
999-1005
|
| 公開日 |
2013-6-1
|
| DOI |
10.4315/0362-028X.JFP-12-543
|
| PMID |
23726195
|
| MeSH |
Beverages / microbiology*
Chaetomium* / drug effects
Chaetomium* / isolation & purification
Colony Count, Microbial
DNA Primers
Drug Resistance, Fungal*
Food Contamination / analysis*
Food Preservation
Food Preservatives / pharmacology*
Humans
Peracetic Acid / pharmacology*
Polymerase Chain Reaction / methods
Spores, Fungal
Tubulin / genetics
|
| IF |
1.581
|
| 引用数 |
8
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 40868
IFM 40869
IFM 40871
IFM 40873
IFM 57191
IFM 57278
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IFM 57280
IFM 57289
IFM 58428
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IFM 58581
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IFM 58824
IFM 58825
IFM 58828
IFM 58830
IFM 58831
IFM 61022
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IFM 61027
IFM 61078
IFM 61079
IFM 61080
IFM 61081
IFM 61082
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IFM 61085
IFM 61086
IFM 61087
IFM 61088
IFM 61089
IFM 61090
IFM 61091
IFM 61092 |
