| RRC ID |
31914
|
| Author |
Brummer E, Kurita N, Yosihida S, Nishimura K, Miyaji M.
|
| Title |
Fungistatic activity of human neutrophils against Histoplasma capsulatum: correlation with phagocytosis.
|
| Journal |
J Infect Dis
|
| Abstract |
The interaction of human polymorphonuclear leukocytes (PMNL) and Histoplasma capsulatum was studied. In limiting dilution assays and in a 2-h coculture system, PMNL were ineffective in killing H. capsulatum whereas Candida albicans was readily killed. Although PMNL could not reduce inoculum colony-forming units of H. capsulatum in short-term assays, they were highly fungistatic in 24- (47%-55%) and 72-h (65%-75%) cocultures. Phagocytosis of yeast cells by PMNL was 81.3% +/- 3.7%. Fungistasis persisted even though PMNL viability decreased with time. Fungistatic activity of PMNL was dose dependent. Fungistasis was verified by direct hemocytometer counts of fungal units. In transwell experiments, PMNL contact with H. capsulatum was required for fungistatic activity. Lymphocytes, PMNL lysates, or PMNL supernatants were not fungistatic in this system.
|
| Volume |
164(1)
|
| Pages |
158-62
|
| Published |
1991-7-1
|
| DOI |
10.1093/infdis/164.1.158
|
| PMID |
2056203
|
| MeSH |
Candida albicans / immunology
Cell Survival
Cells, Cultured
Dose-Response Relationship, Immunologic
Histoplasma / immunology*
Humans
Neutrophils / immunology*
Phagocytosis*
|
| IF |
5.022
|
| Times Cited |
25
|
|
WOS Category
|
INFECTIOUS DISEASES
MICROBIOLOGY
IMMUNOLOGY
|
| Resource |
| Pathogenic microorganisms |
IFM 41329
IFM 46159 |
