| RRC ID |
31916
|
| Author |
Brummer E, Kurita N, Yoshida S, Nishimura K, Miyaji M.
|
| Title |
Killing of Histoplasma capsulatum by gamma-interferon-activated human monocyte-derived macrophages: evidence for a superoxide anion-dependent mechanism.
|
| Journal |
J Med Microbiol
|
| Abstract |
The interaction of human macrophages with the yeast form of the thermally dimorphic fungal pathogen, Histoplasma capsulatum, was studied. Macrophages derived from monocytes by culture in vitro for 3 days ingested H. capsulatum, but were neither fungicidal or fungistatic. In contrast, when monocytes were exposed to human recombinant gamma-interferon (gamma-IFN) during their differentiation into macrophages, those macrophages were able to reduce the number of ingested or adherent cfu of H. capsulatum by 44-75% in 2 h. Activation of macrophages for fungicidal activity by gamma-IFN was dose dependent and 500-1000 units ml were optimal. Antibody to gamma-IFN abrogated the gamma-IFN activation process. Killing of H. capsulatum by activated macrophages in 2-h assays could be inhibited by superoxide dismutase but not by sodium azide.
|
| Volume |
35(1)
|
| Pages |
29-34
|
| Published |
1991-7-1
|
| DOI |
10.1099/00222615-35-1-29
|
| PMID |
1649308
|
| MeSH |
Cell Adhesion
Cell Count
Cells, Cultured
Dose-Response Relationship, Drug
Histoplasma / immunology*
Humans
Interferon-gamma / immunology
Interferon-gamma / pharmacology*
Macrophage Activation*
Macrophages / immunology
Macrophages / microbiology*
Monocytes / microbiology
Neutralization Tests
Recombinant Proteins
Superoxide Dismutase / pharmacology
Superoxides / pharmacology*
|
| IF |
2.156
|
| Times Cited |
27
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Pathogenic microorganisms |
IFM 41329
IFM 41614 |
