RRC ID 31924
著者 Kucsera J, Yarita K, Takeo K.
タイトル Simple detection method for distinguishing dead and living yeast colonies.
ジャーナル J Microbiol Methods
Abstract A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 micromol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies.
巻・号 41(1)
ページ 19-21
公開日 2000-6-1
DOI 10.1016/s0167-7012(00)00136-6
PII S0167-7012(00)00136-6
PMID 10856773
MeSH Basidiomycota / cytology* Basidiomycota / drug effects Candida albicans / cytology* Candida albicans / drug effects Coloring Agents / toxicity Gentian Violet / toxicity Methylene Blue / toxicity Rose Bengal / toxicity Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / drug effects Schizosaccharomyces / cytology* Schizosaccharomyces / drug effects Trypan Blue / toxicity
IF 1.707
引用数 52
WOS 分野 BIOCHEMICAL RESEARCH METHODS MICROBIOLOGY
リソース情報
病原微生物 IFM 40009 IFM 40022 IFM 40078 IFM 40190