論文 - 詳細
RRC ID | 31924 |
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著者 | Kucsera J, Yarita K, Takeo K. |
タイトル | Simple detection method for distinguishing dead and living yeast colonies. |
ジャーナル | J Microbiol Methods |
Abstract |
A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 micromol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies. |
巻・号 | 41(1) |
ページ | 19-21 |
公開日 | 2000-6-1 |
DOI | 10.1016/s0167-7012(00)00136-6 |
PII | S0167-7012(00)00136-6 |
PMID | 10856773 |
MeSH | Basidiomycota / cytology* Basidiomycota / drug effects Candida albicans / cytology* Candida albicans / drug effects Coloring Agents / toxicity Gentian Violet / toxicity Methylene Blue / toxicity Rose Bengal / toxicity Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / drug effects Schizosaccharomyces / cytology* Schizosaccharomyces / drug effects Trypan Blue / toxicity |
IF | 1.707 |
引用数 | 52 |
WOS 分野 | BIOCHEMICAL RESEARCH METHODS MICROBIOLOGY |
リソース情報 | |
病原微生物 | IFM 40009 IFM 40022 IFM 40078 IFM 40190 |