論文 - 詳細
| RRC ID | 31960 |
|---|---|
| 著者 | Xie G, Yu Z, Jia D, Jiao R, Deng WM. |
| タイトル | E(y)1/TAF9 mediates the transcriptional output of Notch signaling in Drosophila. |
| ジャーナル | J Cell Sci |
| Abstract |
Transcriptional activation of Notch signaling targets requires the formation of a ternary complex that involves the intracellular domain of the Notch receptor (NICD), DNA-binding protein Suppressor of Hairless [Su(H), RPBJ in mammals] and coactivator Mastermind (Mam). Here, we report that E(y)1/TAF9, a component of the transcription factor TFIID complex, interacts specifically with the NICD-Su(H)-Mam complex to facilitate the transcriptional output of Notch signaling. We identified E(y)1/TAF9 in a large-scale in vivo RNA interference (RNAi) screen for genes that are involved in a Notch-dependent mitotic-to-endocycle transition in Drosophila follicle cells. Knockdown of e(y)1/TAF9 displayed Notch-mutant-like phenotypes and defects in target gene and activity reporter expression in both the follicle cells and wing imaginal discs. Epistatic analyses in these two tissues indicated that E(y)1/TAF9 functions downstream of Notch cleavage. Biochemical studies in S2 cells demonstrated that E(y)1/TAF9 physically interacts with the transcriptional effectors of Notch signaling Su(H) and NICD. Taken together, our data suggest that the association of the NICD-Su(H)-Mastermind complex with E(y)1/TAF9 in response to Notch activation recruits the transcription initiation complex to induce Notch target genes, coupling Notch signaling with the transcription machinery. |
| 巻・号 | 127(Pt 17) |
| ページ | 3830-9 |
| 公開日 | 2014-9-1 |
| DOI | 10.1242/jcs.154583 |
| PII | jcs.154583 |
| PMID | 25015288 |
| PMC | PMC4150066 |
| MeSH | Animals Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Gene Expression Regulation, Developmental / genetics* Phenotype Receptors, Notch / metabolism* Signal Transduction / genetics* TATA-Binding Protein Associated Factors / metabolism* Transcription Factor TFIID / metabolism* Wings, Animal / metabolism |
| IF | 4.573 |
| 引用数 | 7 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 6474R-1 |