RRC ID 31967
Author Umetsu D, Dunst S, Dahmann C.
Title An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
Journal PLoS ONE
Abstract The formation of straight compartment boundaries separating groups of cells with distinct fates and functions is an evolutionarily conserved strategy during animal development. The physical mechanisms that shape compartment boundaries have recently been further elucidated, however, the molecular mechanisms that underlie compartment boundary formation and maintenance remain poorly understood. Here, we report on the outcome of an RNA interference screen aimed at identifying novel genes involved in maintaining the straight shape of the anteroposterior compartment boundary in Drosophila wing imaginal discs. Out of screening 3114 transgenic RNA interference lines targeting a total of 2863 genes, we identified a single novel candidate that interfered with the formation of a straight anteroposterior compartment boundary. Interestingly, the targeted gene encodes for the Eph receptor tyrosine kinase, an evolutionarily conserved family of signal transducers that has previously been shown to be important for maintaining straight compartment boundaries in vertebrate embryos. Our results identify a hitherto unknown role of the Eph receptor tyrosine kinase in Drosophila and suggest that Eph receptors have important functions in shaping compartment boundaries in both vertebrate and insect development.
Volume 9(12)
Pages e114340
Published 2014
DOI 10.1371/journal.pone.0114340
PII PONE-D-14-10193
PMID 25473846
PMC PMC4256218
MeSH Animals Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / anatomy & histology Drosophila melanogaster / embryology Drosophila melanogaster / genetics* Embryo, Nonmammalian / anatomy & histology Embryo, Nonmammalian / physiology Embryonic Development Gene Knockdown Techniques Imaginal Discs / anatomy & histology Imaginal Discs / embryology Imaginal Discs / metabolism RNA Interference Receptor, EphA1 / genetics* Receptor, EphA1 / metabolism
IF 2.806
Times Cited 7
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Drosophila 8896R-2 3705R-1 9749R-2 7978R-3 18290R-3 12131R-1 5032R-1 2987R-3 2987R-2 10478R-1 17988R-1 31843R-1 12611R-2 8376R-1 1451R-1 1451R-3 1462R-1 1200R-1 10001R-1 9901R-1 9901R-2 7558R-1 7846R-2 10954R-1 10954R-4 5972R-1 5972R-3 5972R-1 6579R-1 8189R-1 32234R-1 8224R-1 3274R-1 6546R-1 5055R-1 5055R-2 7644R-1 4838R-2 14334R-2 10134R-1 14064R-1 18642R-1 10173R-1 12327R-1 5249R-2 3897R-3 8285R-1 9653R-2 13762R-1 5680R-1 3599R-2 6977R-4 14900R-1 10244R-1 6577R-2 4427R-1 30106R-3 7212R-2 31811R-1 10006R-6 10011R-3 10096R-1 10168R-1 10182R-3 10219R-1 10226R-2 10311R-1 10337R-1 10338R-1 10345R-2 10366R-4 10440R-3 10444R-1 10465R-1 10470R-1 10505R-1 10590R-1 10616R-1 10671R-1 10752R-1 10764R-1 10830R-3 10866R-2 10880R-1 10883R-1 1092R-1 10920R-2 11137R-2 11190R-2 11208R-1 11210R-2 11262R-1 11318R-1 11340R-3 11379R-1 11426R-1 11438R-2 11498R-1 11562R-1 11576R-1 11588R-1 11737R-1 11753R-1 11768R-3 11843R-2 11898R-2 11977R-1 12012R-1 12034R-1 12035R-3 12125R-2 12159R-1 12209R-1 12263R-1 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