Reference - Detail
| RRC ID | 31980 |
|---|---|
| Author | Kim SN, Jeibmann A, Halama K, Witte HT, Wälte M, Matzat T, Schillers H, Faber C, Senner V, Paulus W, Klämbt C. |
| Title | ECM stiffness regulates glial migration in Drosophila and mammalian glioma models. |
| Journal | Development |
| Abstract |
Cell migration is an important feature of glial cells. Here, we used the Drosophila eye disc to decipher the molecular network controlling glial migration. We stimulated glial motility by pan-glial PDGF receptor (PVR) activation and identified several genes acting downstream of PVR. Drosophila lox is a non-essential gene encoding a secreted protein that stiffens the extracellular matrix (ECM). Glial-specific knockdown of Integrin results in ECM softening. Moreover, we show that lox expression is regulated by Integrin signaling and vice versa, suggesting that a positive-feedback loop ensures a rigid ECM in the vicinity of migrating cells. The general implication of this model was tested in a mammalian glioma model, where a Lox-specific inhibitor unraveled a clear impact of ECM rigidity in glioma cell migration. |
| Volume | 141(16) |
| Pages | 3233-42 |
| Published | 2014-8-1 |
| DOI | 10.1242/dev.106039 |
| PII | dev.106039 |
| PMID | 25063458 |
| MeSH | Animals Animals, Genetically Modified Base Sequence Cell Line, Tumor Cell Movement Compound Eye, Arthropod / embryology* Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / metabolism Drosophila melanogaster / physiology* Extracellular Matrix / metabolism Extracellular Matrix / physiology* Female Gene Expression Regulation, Developmental Glioblastoma / metabolism Humans Integrins / metabolism Mice Mice, Nude Molecular Sequence Data Neoplasm Transplantation Neuroglia / cytology* Protein-Lysine 6-Oxidase / genetics Protein-Lysine 6-Oxidase / physiology* Signal Transduction |
| IF | 5.611 |
| Times Cited | 38 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Resource | |
| Drosophila | 11335R-3 |