論文 - 詳細
| RRC ID | 31993 |
|---|---|
| 著者 | Kodama Y, Fujishima M. |
| タイトル | Symbiotic Chlorella variabilis incubated under constant dark conditions for 24 hours loses the ability to avoid digestion by host lysosomal enzymes in digestive vacuoles of host ciliate Paramecium bursaria. |
| ジャーナル | FEMS Microbiol Ecol |
| Abstract |
Endosymbiosis between symbiotic Chlorella and alga-free Paramecium bursaria cells can be induced by mixing them. To establish the endosymbiosis, algae must acquire temporary resistance to the host lysosomal enzymes in the digestive vacuoles (DVs). When symbiotic algae isolated from the alga-bearing paramecia are kept under a constant dark conditions for 24 h before mixing with the alga-free paramecia, almost all algae are digested in the host DVs. To examine the cause of algal acquisition to the host lysosomal enzymes, the isolated algae were kept under a constant light conditions with or without a photosynthesis inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea for 24 h, and were mixed with alga-free paramecia. Unexpectedly, most of the algae were not digested in the DVs irrespective of the presence of the inhibitor. Addition of 1 mM maltose, a main photosynthetic product of the symbiotic algae or of a supernatant of the isolated algae kept for 24 h under a constant light conditions, did not rescue the algal digestion in the DVs. These observations reveal that unknown factors induced by light are a prerequisite for algal resistance to the host lysosomal enzymes. |
| 巻・号 | 90(3) |
| ページ | 946-55 |
| 公開日 | 2014-12-8 |
| DOI | 10.1111/1574-6941.12448 |
| PMID | 25348325 |
| MeSH | Chlorella / metabolism* Chlorella / parasitology* Darkness* Diuron / pharmacology Lysosomes / enzymology* Lysosomes / metabolism Maltose / pharmacology Paramecium / metabolism* Photosynthesis / drug effects Photosynthesis / physiology* Symbiosis* |
| IF | 3.675 |
| 引用数 | 13 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゾウリムシ | Yad1w (PB031012B) Yad1g1N (PB031010B) |