論文 - 詳細
| RRC ID | 31997 |
|---|---|
| 著者 | Zheng DM, Bian Z, Furuya N, Oliva Trejo JA, Takeda-Ezaki M, Takahashi K, Hiraoka Y, Mineki R, Taka H, Ikeda S, Komatsu M, Fujimura T, Ueno T, Ezaki J. |
| タイトル | A treadmill exercise reactivates the signaling of the mammalian target of rapamycin (mTor) in the skeletal muscles of starved mice. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
It has been well established that a starvation-induced decrease in insulin/IGF-I and serum amino acids effectively suppresses the mammalian target of rapamycin (mTor) signaling to induce autophagy, which is a major degradative cellular pathway in skeletal muscles. In this study, we investigated the systematic effects of exercise on the mTor signaling of skeletal muscles. Wild type C57BL/6J mice were starved for 24h under synchronous autophagy induction conditions. Under these conditions, endogenous LC3-II increased, while both S6-kinse and S6 ribosomal protein were dephosphorylated in the skeletal muscles, which indicated mTor inactivation. Using GFP-LC3 transgenic mice, it was also confirmed that fluorescent GFP-LC3 dots in the skeletal muscles increased, including soleus, plantaris, and gastrocnemius, which clearly showed autophagosomal induction. These starved mice were then subjected to a single bout of running on a treadmill (12m/min, 2h, with a lean of 10 degrees). Surprisingly, biochemical analyses revealed that the exercise elicited a decrease in the LC3-II/LC3-I ratio as well as an inversion from the dephosphorylated state to the rephosphorylated state of S6-kinase and ribosomal S6 in these skeletal muscles. Consistently, the GFP-LC3 dots of the skeletal muscles were diminished immediately after the exercise. These results indicated that exercise suppressed starvation-induced autophagy through a reactivation of mTor signaling in the skeletal muscles of these starved mice. |
| 巻・号 | 456(1) |
| ページ | 519-26 |
| 公開日 | 2015-1-2 |
| DOI | 10.1016/j.bbrc.2014.11.118 |
| PII | S0006-291X(14)02160-3 |
| PMID | 25485704 |
| MeSH | Animals Autophagy Mice Mice, Inbred C57BL Mice, Transgenic Microtubule-Associated Proteins / metabolism Muscle, Skeletal / metabolism Phosphorylation Physical Conditioning, Animal* Ribosomal Protein S6 Kinases / metabolism Running Signal Transduction Starvation TOR Serine-Threonine Kinases / metabolism* |
| IF | 2.985 |
| 引用数 | 10 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |