論文 - 詳細
| RRC ID | 3203 |
|---|---|
| 著者 | Fujikawa N, Kurumizaka H, Yamazoe M, Hiraga S, Yokoyama S. |
| タイトル | Identification of functional domains of the Escherichia coli SeqA protein. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The Escherichia coli SeqA protein, a negative regulator of chromosomal DNA replication, prevents the overinitiation of replication within one cell cycle by binding to hemimethylated G-mA-T-C sequences in the replication origin, oriC. In addition to the hemimethylated DNA-binding activity, the SeqA protein has a self-association activity, which is also considered to be essential for its regulatory function in replication initiation. To study the functional domains responsible for the DNA-binding and self-association activities, we performed a deletion analysis of the SeqA protein and found that the N-terminal (amino acid residues 1-59) and the C-terminal (amino acid residues 71-181) regions form structurally distinct domains. The N-terminal domain, which is not involved in DNA binding, has the self-association activity. In contrast, the C-terminal domain, which lacks the self-association activity, specifically binds to the hemimethylated G-mA-T-C sequence. Therefore, two essential SeqA activities, self-association and DNA-binding, are independently performed by the structurally distinct N-terminal and C-terminal domains, respectively. |
| 巻・号 | 300(3) |
| ページ | 699-705 |
| 公開日 | 2003-1-17 |
| DOI | 10.1016/s0006-291x(02)02891-7 |
| PII | S0006291X02028917 |
| PMID | 12507506 |
| MeSH | Bacterial Outer Membrane Proteins Chromatography, Gel DNA / metabolism DNA Methylation DNA Replication / physiology DNA-Binding Proteins Escherichia coli Escherichia coli Proteins Mutagenesis, Site-Directed Protein Structure, Tertiary / physiology Sequence Deletion Structure-Activity Relationship Transcription Factors / chemistry* Transcription Factors / genetics* |
| IF | 2.985 |
| 引用数 | 8 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | CV-25(DH5alpha)? |