論文 - 詳細
| RRC ID | 32172 |
|---|---|
| 著者 | Sawai S, Ohyama K, Yasumoto S, Seki H, Sakuma T, Yamamoto T, Takebayashi Y, Kojima M, Sakakibara H, Aoki T, Muranaka T, Saito K, Umemoto N. |
| タイトル | Sterol side chain reductase 2 is a key enzyme in the biosynthesis of cholesterol, the common precursor of toxic steroidal glycoalkaloids in potato. |
| ジャーナル | Plant Cell |
| Abstract |
Potatoes (Solanum tuberosum) contain α-solanine and α-chaconine, two well-known toxic steroidal glycoalkaloids (SGAs). Sprouts and green tubers accumulate especially high levels of SGAs. Although SGAs were proposed to be biosynthesized from cholesterol, the biosynthetic pathway for plant cholesterol is poorly understood. Here, we identify sterol side chain reductase 2 (SSR2) from potato as a key enzyme in the biosynthesis of cholesterol and related SGAs. Using in vitro enzyme activity assays, we determined that potato SSR2 (St SSR2) reduces desmosterol and cycloartenol to cholesterol and cycloartanol, respectively. These reduction steps are branch points in the biosynthetic pathways between C-24 alkylsterols and cholesterol in potato. Similar enzymatic results were also obtained from tomato SSR2. St SSR2-silenced potatoes or St SSR2-disrupted potato generated by targeted genome editing had significantly lower levels of cholesterol and SGAs without affecting plant growth. Our results suggest that St SSR2 is a promising target gene for breeding potatoes with low SGA levels. |
| 巻・号 | 26(9) |
| ページ | 3763-74 |
| 公開日 | 2014-9-1 |
| DOI | 10.1105/tpc.114.130096 |
| PII | tpc.114.130096 |
| PMID | 25217510 |
| PMC | PMC4213163 |
| MeSH | Alkaloids / chemistry Alkaloids / metabolism* Biosynthetic Pathways* Cholesterol / analogs & derivatives Cholesterol / biosynthesis* Cholesterol / chemistry Enzyme Assays Gene Silencing Genes, Plant Molecular Sequence Data Oxidoreductases / metabolism Phylogeny Phytosterols / biosynthesis Phytosterols / chemistry RNA Editing Solanum lycopersicum / genetics Solanum tuberosum / enzymology* Solanum tuberosum / genetics Tetraploidy |
| IF | 9.618 |
| 引用数 | 89 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 26 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| トマト | TOMJPF00001 |