RRC ID 32187
Author Mijiti J, Pu XM, Erfan A, Yaguchi T, Chibana H, Tanaka R.
Title Genotyping of fluconazole-resistant Candida albicans isolated from Uighurian people in Xinjing (China) using ALTS/RFLP and micro-TGGE method.
Journal Nihon Ishinkin Gakkai Zasshi
Abstract Antifungal susceptibility tests were performed for 38 Candida albicans strains isolated from oral cavities of 43 Uighurian AIDS patients. Results showed that six isolates were resistant to fluconazole; one showed low susceptibility. We attempted to examine these strains molecular-epidemiologically, but 25S rDNA genotyping was insufficient for their discrimination. To estimate whether the origins of resistant strains were identical, we developed a new combination method of C. albicans tandem repeating units (ALTS)/RFLP and micro-temperature gradient gel electrophoresis (micro-TGGE). This new method was able to distinguish all seven strains. A suspected nosocomial infection was ruled out.
Volume 51(3)
Pages 165-8
Published 2010
PII JST.JSTAGE/jjmm/51.165
PMID 20716855
MeSH Acquired Immunodeficiency Syndrome / microbiology Antifungal Agents / pharmacology* Candida albicans / drug effects* Candida albicans / genetics Candida albicans / isolation & purification* China Denaturing Gradient Gel Electrophoresis Fluconazole / pharmacology* Genotype Humans Molecular Epidemiology Polymorphism, Restriction Fragment Length Tandem Repeat Sequences
Pathogenic microorganisms IFM 57372 IFM 57373 IFM 57374 IFM 57376 IFM 57377 IFM 57378 IFM 57379 IFM 57380 IFM 57381 IFM 57382 IFM 57383 IFM 57384 IFM 57386 IFM 57387 IFM 57388 IFM 57389 IFM 57390 IFM 57391 IFM 57392 IFM 57398 IFM 57400 IFM 57402 IFM 57403 IFM 57404 IFM 57406 IFM 57407 IFM 57408 IFM 57409 IFM 57410 IFM 57411 IFM 57415 IFM 57416 IFM 57417 IFM 57418 IFM 57419 IFM 57420 IFM 57421 IFM 57422