論文 - 詳細
| RRC ID | 32192 |
|---|---|
| 著者 | Wang L, Yokoyama K, Miyaji M, Nishimura K. |
| タイトル | The identification and phylogenetic relationship of pathogenic species of Aspergillus based on the mitochondrial cytochrome b gene. |
| ジャーナル | Med Mycol |
| Abstract |
To study the identification and phylogeny of pathogenic isolates of Aspergillus, we designed primers from known cytochrome b amino acid sequences. Using these primers, 426 bp fragments of a mitochondrial (mt) cytochrome b gene were amplified by polymerase chain reaction (PCR), directly sequenced, and compared among Aspergillus fumigatus, A. flavus, A. niger, A. terreus and Emericella nidulans. Except for E. nidulans, all strains produced the 426 bp fragment by PCR. The E. nidulans strains demonstrated both an intron-presence fragment ( approximately 1500 bp) and intron-absence fragment (426 bp). Species-specific nucleotides were found in each of the five species. Based on sequence analysis, the strains were further divided into several groups within each species. When a 142-amino-acid sequence was estimated from the 426 bp nucleotide sequence using the yeast mt genetic code, the amino acid sequences showed no difference among strains of the individual species. DNA-based phylogenetic and amino acid-based trees were constructed. In conclusion, the DNA sequences of the cytochrome b gene may be of use in identification of pathogenic Aspergillus species and the amino acid-based tree suitable for discussing their phylogenetic relationships. |
| 巻・号 | 36(3) |
| ページ | 153-64 |
| 公開日 | 1998-6-1 |
| PMID | 9776828 |
| MeSH | Amino Acid Sequence Aspergillus / classification Aspergillus / genetics* Aspergillus / pathogenicity Aspergillus flavus / genetics Aspergillus fumigatus / genetics Aspergillus niger / genetics Base Sequence Cytochrome b Group / genetics* DNA, Fungal / genetics DNA, Mitochondrial / genetics* Genes, Fungal Introns Molecular Sequence Data Phylogeny* Plants / genetics Polymerase Chain Reaction Sequence Alignment Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Virulence |
| IF | 2.822 |
| 引用数 | 27 |
| WOS 分野 | VETERINARY SCIENCES MYCOLOGY INFECTIOUS DISEASES |
| リソース情報 | |
| 病原微生物 | IFM 5355 IFM 5366 IFM 5367 IFM 5368 IFM 5372 IFM 40603 IFM 40606 IFM 40800 IFM 40804 IFM 40806 IFM 40807 IFM 40819 IFM 40838 IFM 40850 IFM 40851 IFM 40852 IFM 41087 IFM 41092 IFM 41094 IFM 41206 IFM 41392 IFM 41395 IFM 41398 IFM 41399 IFM 41933 IFM 45909 IFM 45910 IFM 45911 IFM 45916 IFM 45917 IFM 46870 IFM 46897 IFM 46980 IFM 46997 IFM 46998 IFM 46999 IFM 47001 IFM 47002 IFM 47003 IFM 47005 IFM 47006 |