論文 - 詳細
| RRC ID | 32204 |
|---|---|
| 著者 | Hanafy A, Kaocharoen S, Jover-Botella A, Katsu M, Iida S, Kogure T, Gonoi T, Mikami Y, Meyer W. |
| タイトル | Multilocus microsatellite typing for Cryptococcus neoformans var. grubii. |
| ジャーナル | Med Mycol |
| Abstract |
Fifteen randomly selected microsatellites (simple sequence repeats; SSRs), from the H99 Cryptococcus neoformans var. grubii (serotype A) genome, were sequenced, characterized and applied to sequence 87 clinical and environmental C. neoformans var. grubii isolates from 12 different countries based on Multilocus Microsatellite Typing (MLMT). Among the 15 SSR loci, three (designated CNG1, CNG2 and CNG3) were polymorphic, while the remaining 12 SSR loci showed no variations. The specific PCR primers of the polymorphic microsatellites, i.e., CNG1, CNG2 and CNG3, amplified those loci only from strains of C. neoformans (C. neoformans var. grubii, C. neoformans var. neoformans and the AD hybrid) but not from Cryptococcus gattii, suggesting a species-specific association. The three polymorphic microsatellites are useful markers for strain genotyping, population genetic analysis, epidemiological studies, and may be helpful for the diagnosis of cryptococcosis due to C. neoformans. |
| 巻・号 | 46(7) |
| ページ | 685-96 |
| 公開日 | 2008-11-1 |
| DOI | 10.1080/13693780802027062 |
| PII | 793159243 |
| PMID | 18608927 |
| MeSH | Base Sequence Cryptococcus neoformans / genetics* Electrophoresis, Polyacrylamide Gel Genetic Variation Genome, Fungal / genetics Geography Microsatellite Repeats / genetics* Molecular Sequence Data Mycological Typing Techniques / methods* Polymorphism, Genetic Sequence Alignment Serotyping Species Specificity |
| IF | 2.822 |
| 引用数 | 28 |
| WOS 分野 | VETERINARY SCIENCES MYCOLOGY INFECTIOUS DISEASES |
| リソース情報 | |
| 病原微生物 | IFM 5807 IFM 5808 IFM 5814 IFM 5874 IFM 5876 IFM 5877 IFM 5888 IFM 10177 IFM 10201 IFM 10271 IFM 40216 IFM 45705 IFM 45718 IFM 45728 IFM 45729 IFM 45732 IFM 45735 IFM 45923 IFM 45933 IFM 45981 IFM 45985 IFM 46501 IFM 46502 IFM 46503 IFM 46504 IFM 46515 IFM 46531 IFM 46551 IFM 46553 IFM 46563 IFM 46641 IFM 46652 IFM 46653 IFM 46660 IFM 46661 IFM 46664 IFM 46665 IFM 46670 IFM 46706 IFM 46713 IFM 46716 IFM 46728 IFM 46736 IFM 46760 IFM 47274 IFM 48224 IFM 48225 IFM 48229 IFM 48231 IFM 48235 IFM 48254 IFM 48255 IFM 48256 IFM 48257 IFM 48259 IFM 48260 IFM 48261 IFM 48263 IFM 48264 IFM 48265 IFM 48274 IFM 48279 IFM 48282 IFM 48283 IFM 48284 IFM 48286 IFM 48287 IFM 48293 IFM 48294 IFM 48295 IFM 48296 IFM 48304 IFM 48810 IFM 48812 IFM 48813 IFM 52965 IFM 52966 IFM 52967 IFM 52969 IFM 52970 IFM 52971 IFM 52972 |