RRC ID 32216
Author Nakamura J, Yoshida K, Sasakura Y, Fujiwara S.
Title Chondroitin 6-O-sulfotransferases are required for morphogenesis of the notochord in the ascidian embryo.
Journal Dev Dyn
Abstract BACKGROUND:Chondroitin sulfate (CS) is a sulfated polysaccharide chain that binds to various core proteins to form proteoglycans. The amount and position of sulfate groups in CS are variable among different tissues, and are determined by specific sulfotransferases. Although the ascidians are the closest relatives of vertebrates, the functions of their sulfotransferases have not been studied.
RESULTS:The genome of the ascidian Ciona intestinalis contains eight genes encoding proteins similar to chondroitin 6-O-sulfotransferases (C6STs), which appear to have independently diverged in the ascidian lineage during evolution. Among them, Ci-C6ST-like1 and Ci-C6ST-like7 were predominantly expressed in the developing notochord. In addition, they were weakly expressed in the neural tube. The disruption of either one of them affected the convergent extension movement of notochordal cells. Presumptive notochord cells coming from both sides of the embryo did not intercalate. The results suggest that both of them are necessary. In some cases, the anterior neural tube failed to close. Forced expression of Ci-C6ST-like1 or Ci-C6ST-like7 in the notochord restored the normal intercalation of notochordal cells, indicating that the effects of morpholino oligos are specific.
CONCLUSIONS:Ci-C6ST-like1 and Ci-C6ST-like7 are required for the morphogenesis of the notochord in the ascidian embryo.
Volume 243(12)
Pages 1637-45
Published 2014-12-1
DOI 10.1002/dvdy.24213
PMID 25298188
MeSH Animals Ciona intestinalis / cytology Ciona intestinalis / embryology* Ciona intestinalis / genetics Embryo, Nonmammalian / cytology Embryo, Nonmammalian / embryology* Gene Expression Regulation, Developmental / physiology Gene Expression Regulation, Enzymologic / physiology Gene Knockdown Techniques Notochord / cytology Notochord / embryology* Organogenesis / physiology* Sulfotransferases / biosynthesis* Sulfotransferases / genetics
IF 3.275
Times Cited 3
C.intestinalis / (O.japonicus) Wild C. int