RRC ID 32226
Author Yazawa K, Mikami Y, Maeda A, Kudo T, Suzuki K, Saito N, Kubo A.
Title Inactivation of kanamycin A by phosphorylation in pathogenic Nocardia.
Journal Microbiol Immunol
Abstract Among the five species of pathogenic Nocardia, i.e., N. asteroides, N. brasiliensis, N. farcinica, N. nova and N. otitidiscaviarum, all strains of N. brasiliensis and N. farcinica showed resistance to an aminoglycoside antibiotic, kanamycin A, showing the MIC (minimum inhibitory concentration) values of more than 100 micrograms/ml. This species-specific difference in sensitivity was found to be explained by the production of an inactivation enzyme, aminoglycoside 3'-phosphotransferase APH(3'). Structural studies by mass and NMR spectroscopy on the inactivated substance produced by a cell-free extract of the Nocardia confirmed the conversion of kanamycin A to an inactive substance, kanamycin A 3'-phosphate. The MIC values of N. otitidiscaviarum and N. nova for kanamycin A, on the other hand, ranged from 0.78 micrograms/ml to 100 micrograms/ml, and both species were non-producers of APH(3'). Sensitivity to the antibiotic and APH(3') productivity of N. asteroides varied depending on the strain.
Volume 35(1)
Pages 39-48
Published 1991-1-1
DOI 10.1111/j.1348-0421.1991.tb01531.x
PMID 1651439
MeSH Carbohydrate Sequence Enzyme Activation Kanamycin / metabolism* Kanamycin Kinase Kanamycin Resistance Magnetic Resonance Spectroscopy Mass Spectrometry Microbial Sensitivity Tests Molecular Sequence Data Nocardia / drug effects Nocardia / metabolism* Phosphorylation Phosphotransferases / metabolism* Species Specificity
IF 1.566
Times Cited 6
Pathogenic microorganisms IFM 0001 IFM 0042 IFM 0044 IFM 0091 IFM 0092 IFM 0150 IFM 0225 IFM 0228 IFM 0230 IFM 0233 IFM 0234 IFM 0235 IFM 0236 IFM 0239 IFM 0246 IFM 0247 IFM 0248 IFM 0251 IFM 0252 IFM 0253 IFM 0275 IFM 0284 IFM 0290 IFM 0304 IFM 0307 IFM 0311 IFM 0315 IFM 0316 IFM 0317 IFM 0318 IFM 0319