論文 - 詳細
| RRC ID | 32269 |
|---|---|
| 著者 | Ozaki S, Matsuda Y, Keyamura K, Kawakami H, Noguchi Y, Kasho K, Nagata K, Masuda T, Sakiyama Y, Katayama T. |
| タイトル | A replicase clamp-binding dynamin-like protein promotes colocalization of nascent DNA strands and equipartitioning of chromosomes in E. coli. |
| ジャーナル | Cell Rep |
| Abstract |
In Escherichia coli, bidirectional chromosomal replication is accompanied by the colocalization of sister replication forks. However, the biological significance of this mechanism and the key factors involved are still largely unknown. In this study, we found that a protein, termed CrfC, helps sustain the colocalization of nascent DNA regions of sister replisomes and promote chromosome equipartitioning. CrfC formed homomultimers that bound to multiple molecules of the clamp, a replisome subunit that encircles DNA, and colocalized with nascent DNA regions in a clamp-binding-dependent manner in living cells. CrfC is a dynamin homolog; however, it lacks the typical membrane-binding moiety and instead possesses a clamp-binding motif. Given that clamps remain bound to DNA after Okazaki fragment synthesis, we suggest that CrfC sustains the colocalization of sister replication forks in a unique manner by linking together the clamp-loaded nascent DNA strands, thereby laying the basis for subsequent chromosome equipartitioning. |
| 巻・号 | 4(5) |
| ページ | 985-95 |
| 公開日 | 2013-9-12 |
| DOI | 10.1016/j.celrep.2013.07.040 |
| PII | S2211-1247(13)00402-6 |
| PMID | 23994470 |
| MeSH | Amino Acid Sequence Chromosome Segregation Chromosomes, Bacterial* DNA Helicases / genetics DNA Helicases / metabolism* DNA Replication / physiology DNA, Bacterial / genetics DNA, Bacterial / metabolism* Dynamins / genetics Dynamins / metabolism* Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli / metabolism* Molecular Sequence Data Trans-Activators / genetics Trans-Activators / metabolism* |
| IF | 8.109 |
| 引用数 | 16 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection |