| RRC ID |
3237
|
| Author |
Ito M, Baba T, Mori H, Mori H.
|
| Title |
Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays.
|
| Journal |
Metab Eng
|
| Abstract |
Escherichia coli is one of the best elucidated organisms. However, about 40% of E. coli genes have not been assigned to their function yet. We analyzed 1440 single gene knock-out mutants using the GN2-MicroPlate, which permits assay of 95 carbon-source utilizations simultaneously. In the knock-out library there are 1044 of so called y-genes with no apparent function. The raw dataset was analyzed and genes were interrelated by the clustering method of the GeneSpring software. In the resulted dendrogram of genes, a group of genes with known and related function tended to be assembled into a cluster. Our clustering method would be useful for functional assignment of so called y-genes with no apparent function, since the resulted dendrogram could connect y-genes to phenotype and function of well-studied genes.
|
| Volume |
7(4)
|
| Pages |
318-27
|
| Published |
2005-7-1
|
| DOI |
10.1016/j.ymben.2005.06.004
|
| PII |
S1096-7176(05)00049-2
|
| PMID |
16095938
|
| MeSH |
Escherichia coli / genetics*
Gene Deletion*
Gene Expression Profiling
Gene Expression Regulation, Bacterial / genetics
Genes, Bacterial / genetics*
Oligonucleotide Array Sequence Analysis / methods
Phenotype*
Software*
|
| IF |
7.263
|
| Times Cited |
31
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Prokaryotes E. coli |
Keio collection |
