RRC ID 32387
Author Kim MJ, Choe KM.
Title Basement membrane and cell integrity of self-tissues in maintaining Drosophila immunological tolerance.
Journal PLoS Genet
Abstract The mechanism underlying immune system recognition of different types of pathogens has been extensively studied over the past few decades; however, the mechanism by which healthy self-tissue evades an attack by its own immune system is less well-understood. Here, we established an autoimmune model of melanotic mass formation in Drosophila by genetically disrupting the basement membrane. We found that the basement membrane endows otherwise susceptible target tissues with self-tolerance that prevents autoimmunity, and further demonstrated that laminin is a key component for both structural maintenance and the self-tolerance checkpoint function of the basement membrane. Moreover, we found that cell integrity, as determined by cell-cell interaction and apicobasal polarity, functions as a second discrete checkpoint. Target tissues became vulnerable to blood cell encapsulation and subsequent melanization only after loss of both the basement membrane and cell integrity.
Volume 10(10)
Pages e1004683
Published 2014-10-1
DOI 10.1371/journal.pgen.1004683
PMID 25329560
PMC PMC4199487
MeSH Animals Animals, Genetically Modified Autoimmunity / genetics Basement Membrane / cytology* Basement Membrane / immunology Cell Communication Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / immunology* Immune Tolerance* Laminin / genetics Laminin / metabolism Larva / immunology RNA Interference
IF 5.224
Times Cited 8
Drosophila 16858R-3 12497R-1 12908R-3 1771R-1 16827R-2 1762R-1 1560R-1 8470R-4 11990R-2 1725R-1 5803R-1