| RRC ID |
32392
|
| 著者 |
Zobel T, Brinkmann K, Koch N, Schneider K, Seemann E, Fleige A, Qualmann B, Kessels MM, Bogdan S.
|
| タイトル |
Cooperative functions of the two F-BAR proteins Cip4 and Nostrin in the regulation of E-cadherin in epithelial morphogenesis.
|
| ジャーナル |
J Cell Sci
|
| Abstract |
F-BAR proteins are prime candidates to regulate membrane curvature and dynamics during different developmental processes. Here, we analyzed nostrin, a so-far-unknown Drosophila melanogaster F-BAR protein related to Cip4. Genetic analyses revealed a strong synergism between nostrin and cip4 functions.Whereas single mutant flies are viable and fertile, combined loss of nostrin and cip4 results in reduced viability and fertility. Double mutant escaper flies show enhanced wing polarization defects and females exhibit strong egg chamber encapsulation defects. Live imaging analysis suggests that the observed phenotypes are caused by an impaired turnover of E-cadherin at the membrane. Simultaneous knockdown of Cip4 and Nostrin strongly increases the formation of tubular E-cadherin vesicles at adherens junctions. Cip4 and Nostrin localize at distinct membrane subdomains. Both proteins prefer similar membrane curvatures but seem to form distinct membrane coats and do not heterooligomerize. Our data suggest an important synergistic function of both F-BAR proteins in membrane dynamics. We propose a cooperative recruitment model, in which Cip4 initially promotes membrane invagination and early-actin-based endosomal motility, and Nostrin makes contacts with microtubules through the kinesin Khc-73 for trafficking of recycling endosomes.
|
| 巻・号 |
128(3)
|
| ページ |
499-515
|
| 公開日 |
2015-2-1
|
| DOI |
10.1242/jcs.155929
|
| PII |
jcs.155929
|
| PMID |
25413347
|
| MeSH |
Adherens Junctions / metabolism
Animals
Cadherins / metabolism*
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cell Differentiation
Cell Line
Drosophila Proteins / genetics
Drosophila Proteins / metabolism*
Drosophila melanogaster / embryology
Drosophila melanogaster / genetics
Endocytosis / genetics
Endocytosis / physiology
Endosomes / metabolism
Epithelial Cells / cytology
Kinesins / metabolism
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / metabolism*
Microtubules / metabolism*
Morphogenesis / physiology
Ovum / physiology*
Protein Transport / physiology
RNA Interference
RNA, Small Interfering
Wings, Animal / embryology*
|
| IF |
4.573
|
| 引用数 |
12
|
|
WOS 分野
|
CELL BIOLOGY
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| リソース情報 |
| ショウジョウバエ |
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