RRC ID 32392
Author Zobel T, Brinkmann K, Koch N, Schneider K, Seemann E, Fleige A, Qualmann B, Kessels MM, Bogdan S.
Title Cooperative functions of the two F-BAR proteins Cip4 and Nostrin in the regulation of E-cadherin in epithelial morphogenesis.
Journal J. Cell. Sci.
Abstract F-BAR proteins are prime candidates to regulate membrane curvature and dynamics during different developmental processes. Here, we analyzed nostrin, a so-far-unknown Drosophila melanogaster F-BAR protein related to Cip4. Genetic analyses revealed a strong synergism between nostrin and cip4 functions.Whereas single mutant flies are viable and fertile, combined loss of nostrin and cip4 results in reduced viability and fertility. Double mutant escaper flies show enhanced wing polarization defects and females exhibit strong egg chamber encapsulation defects. Live imaging analysis suggests that the observed phenotypes are caused by an impaired turnover of E-cadherin at the membrane. Simultaneous knockdown of Cip4 and Nostrin strongly increases the formation of tubular E-cadherin vesicles at adherens junctions. Cip4 and Nostrin localize at distinct membrane subdomains. Both proteins prefer similar membrane curvatures but seem to form distinct membrane coats and do not heterooligomerize. Our data suggest an important synergistic function of both F-BAR proteins in membrane dynamics. We propose a cooperative recruitment model, in which Cip4 initially promotes membrane invagination and early-actin-based endosomal motility, and Nostrin makes contacts with microtubules through the kinesin Khc-73 for trafficking of recycling endosomes.
Volume 128(3)
Pages 499-515
Published 2015-2-1
DOI 10.1242/jcs.155929
PII jcs.155929
PMID 25413347
MeSH Adherens Junctions / metabolism Animals Cadherins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Differentiation Cell Line Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / embryology Drosophila melanogaster / genetics Endocytosis / genetics Endocytosis / physiology Endosomes / metabolism Epithelial Cells / cytology Kinesin / metabolism Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism* Microtubules / metabolism* Morphogenesis / physiology Ovum / physiology* Protein Transport / physiology RNA Interference RNA, Small Interfering Wings, Animal / embryology*
IF 4.401
Times Cited 6