RRC ID 32432
Author Joshi SS, Cheong H, Meller VH.
Title A strategy for generation and balancing of autosome: Y chromosome translocations.
Journal Fly (Austin)
Abstract We describe a method for generation and maintenance of translocations that move large autosomal segments onto the Y chromosome. Using this strategy we produced ( 2;Y) translocations that relocate between 1.5 and 4.8 Mb of the 2nd chromosome.. All translocations were easily balanced over a male-specific lethal 1 (msl-1) mutant chromosome. Both halves of the translocation carry visible markers, as well as P-element ends that enable molecular confirmation. Halves of these translocations can be separated to produce offspring with duplications and with lethal second chromosome deficiencies . Such large deficiencies are otherwise tedious to generate and maintain.
Volume 8(1)
Pages 58-62
Published 2014-1-16
DOI 10.4161/fly.27814
PII 27814
PMID 24646827
PMC PMC3974896
MeSH Animals Chromosomes, Insect* Drosophila / genetics* Female Genetic Techniques* Male Translocation, Genetic* Y Chromosome*
IF 1.204
Times Cited 1
Drosophila DGRC#125639 DGRC#124255 DGRC#124291 DGRC#124216 DGRC#123609