論文 - 詳細
| RRC ID | 32442 |
|---|---|
| 著者 | Dong B, Miao G, Hayashi S. |
| タイトル | A fat body-derived apical extracellular matrix enzyme is transported to the tracheal lumen and is required for tube morphogenesis in Drosophila. |
| ジャーナル | Development |
| Abstract |
The apical extracellular matrix plays a central role in epithelial tube morphogenesis. In the Drosophila tracheal system, Serpentine (Serp), a secreted chitin deacetylase expressed by the tracheal cells plays a key role in regulating tube length. Here, we show that the fly fat body, which is functionally equivalent to the mammalian liver, also contributes to tracheal morphogenesis. Serp was expressed by the fat body, and the secreted Serp was taken up by the tracheal cells and translocated to the lumen to functionally support normal tracheal development. This process was defective in rab9 and shrub/vps32 mutants and in wild-type embryos treated with a secretory pathway inhibitor, leading to an abundant accumulation of Serp in the fat body. We demonstrated that fat body-derived Serp reached the tracheal lumen after establishment of epithelial barrier function and was retained in the lumen in a chitin synthase-dependent manner. Our results thus reveal that the fat body, a mesodermal organ, actively contributes to tracheal development. |
| 巻・号 | 141(21) |
| ページ | 4104-9 |
| 公開日 | 2014-11-1 |
| DOI | 10.1242/dev.109975 |
| PII | 141/21/4104 |
| PMID | 25336738 |
| PMC | PMC4302886 |
| MeSH | Animals Drosophila / embryology* Drosophila / enzymology* Drosophila / metabolism Extracellular Matrix / enzymology* Extracellular Matrix / metabolism* Fat Body / metabolism* Humans Morphogenesis Trachea / embryology* Trachea / enzymology* Trachea / metabolism |
| IF | 5.611 |
| 引用数 | 19 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | UAS-shi K44A svp[1] twi-GAL4 and kkv[1] |