論文 - 詳細
| RRC ID | 32455 |
|---|---|
| 著者 | Liu J, Gong Z, Liu L. |
| タイトル | γ-glutamyl transpeptidase 1 specifically suppresses green-light avoidance via GABAA receptors in Drosophila. |
| ジャーナル | J Neurochem |
| Abstract |
Drosophila larvae innately show light avoidance behavior. Compared with robust blue-light avoidance, larvae exhibit relatively weaker green-light responses. In our previous screening for genes involved in larval light avoidance, compared with control w(1118) larvae, larvae with γ-glutamyl transpeptidase 1 (Ggt-1) knockdown or Ggt-1 mutation were found to exhibit higher percentage of green-light avoidance which was mediated by Rhodopsin6 (Rh6) photoreceptors. However, their responses to blue light did not change significantly. By adjusting the expression level of Ggt-1 in different tissues, we found that Ggt-1 in malpighian tubules was both necessary and sufficient for green-light avoidance. Our results showed that glutamate levels were lower in Ggt-1 null mutants compared with controls. Feeding Ggt-1 null mutants glutamate can normalize green-light avoidance, indicating that high glutamate concentrations suppressed larval green-light avoidance. However, rather than directly, glutamate affected green-light avoidance indirectly through GABA, the level of which was also lower in Ggt-1 mutants compared with controls. Mutants in glutamate decarboxylase 1, which encodes GABA synthase, and knockdown lines of the GABAA receptor, both exhibit elevated levels of green-light avoidance. Thus, our results elucidate the neurobiological mechanisms mediating green-light avoidance, which was inhibited in wild-type larvae. |
| 巻・号 | 130(3) |
| ページ | 408-18 |
| 公開日 | 2014-8-1 |
| DOI | 10.1111/jnc.12735 |
| PMID | 24702462 |
| MeSH | Animals Animals, Genetically Modified Avoidance Learning / drug effects* Blotting, Western Color Drosophila Gene Knockdown Techniques Glutamate Decarboxylase / genetics Glutamate Decarboxylase / physiology Glutamic Acid / metabolism Glutamic Acid / physiology Larva Neurotransmitter Agents / physiology Photic Stimulation Polymerase Chain Reaction RNA Interference Receptors, GABA-A / drug effects* Receptors, GABA-A / genetics gamma-Aminobutyric Acid / metabolism gamma-Glutamyltransferase / genetics* gamma-Glutamyltransferase / physiology* |
| IF | 4.066 |
| 引用数 | 4 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#104556 DGRC#103538 |