| RRC ID |
32486
|
| Author |
Pyo JH, Park JS, Na HJ, Jeon HJ, Lee SH, Kim JG, Park SY, Jin YW, Kim YS, Yoo MA.
|
| Title |
Functional modification of Drosophila intestinal stem cells by ionizing radiation.
|
| Journal |
Radiat Res
|
| Abstract |
Although the diverse effects of ionizing radiation on biological and pathological processes at various levels ranging from molecular to whole body are well studied, the effects on adult stem cells by ionizing radiation remain largely unknown. In this study, we characterized the functional modifications of adult Drosophila midgut intestinal stem cells after ionizing radiation treatment. A dose of 10 Gy of radiation decreased the proliferative capacity of intestinal stem cells. Interestingly, after irradiation at 2 Gy, the intestinal stem cells exhibited increased proliferative activity, misdifferentiation and γH2AvD and 8-oxo-dG levels. In addition, the guts irradiated with 2 Gy showed increased JNK and AKT activities. Furthermore, we showed that 2 Gy of ionizing radiation induced centrosome amplification in intestinal stem cells of adult midguts. Our data gives molecular insights into the effects of ionizing radiation on functional modifications of stem cells. The adult Drosophila midgut intestinal stem cells offer a potentially rich new system for the exploration of the biological effects of ionizing radiation.
|
| Volume |
181(4)
|
| Pages |
376-86
|
| Published |
2014-4-1
|
| DOI |
10.1667/RR13545.1
|
| PMID |
24701963
|
| MeSH |
Animals
Cell Proliferation / radiation effects
Centrosome
DNA Damage
Drosophila
Intestines / cytology
Intestines / radiation effects*
Radiation, Ionizing*
Stem Cells / cytology
Stem Cells / radiation effects*
|
| IF |
2.657
|
| Times Cited |
7
|
|
WOS Category
|
BIOLOGY
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOPHYSICS
|
| Resource |
| Drosophila |
|
