RRC ID 32515
著者 Cavanaugh DJ, Geratowski JD, Wooltorton JR, Spaethling JM, Hector CE, Zheng X, Johnson EC, Eberwine JH, Sehgal A.
タイトル Identification of a circadian output circuit for rest:activity rhythms in Drosophila.
ジャーナル Cell
Abstract Though much is known about the cellular and molecular components of the circadian clock, output pathways that couple clock cells to overt behaviors have not been identified. We conducted a screen for circadian-relevant neurons in the Drosophila brain and report here that cells of the pars intercerebralis (PI), a functional homolog of the mammalian hypothalamus, comprise an important component of the circadian output pathway for rest:activity rhythms. GFP reconstitution across synaptic partners (GRASP) analysis demonstrates that PI cells are connected to the clock through a polysynaptic circuit extending from pacemaker cells to PI neurons. Molecular profiling of relevant PI cells identified the corticotropin-releasing factor (CRF) homolog, DH44, as a circadian output molecule that is specifically expressed by PI neurons and is required for normal rest:activity rhythms. Notably, selective activation or ablation of just six DH44+ PI cells causes arrhythmicity. These findings delineate a circuit through which clock cells can modulate locomotor rhythms.
巻・号 157(3)
ページ 689-701
公開日 2014-4-24
DOI 10.1016/j.cell.2014.02.024
PII S0092-8674(14)00223-2
PMID 24766812
PMC PMC4003459
MeSH Animals Animals, Genetically Modified Brain / cytology Brain / physiology Circadian Clocks* Circadian Rhythm Drosophila / cytology Drosophila / physiology* Neurons / cytology Neurons / physiology* Single-Cell Analysis Transcriptome
IF 38.637
引用数 104
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ショウジョウバエ DGRC#104906 DGRC#112361