論文 - 詳細
| RRC ID | 32515 |
|---|---|
| 著者 | Cavanaugh DJ, Geratowski JD, Wooltorton JR, Spaethling JM, Hector CE, Zheng X, Johnson EC, Eberwine JH, Sehgal A. |
| タイトル | Identification of a circadian output circuit for rest:activity rhythms in Drosophila. |
| ジャーナル | Cell |
| Abstract |
Though much is known about the cellular and molecular components of the circadian clock, output pathways that couple clock cells to overt behaviors have not been identified. We conducted a screen for circadian-relevant neurons in the Drosophila brain and report here that cells of the pars intercerebralis (PI), a functional homolog of the mammalian hypothalamus, comprise an important component of the circadian output pathway for rest:activity rhythms. GFP reconstitution across synaptic partners (GRASP) analysis demonstrates that PI cells are connected to the clock through a polysynaptic circuit extending from pacemaker cells to PI neurons. Molecular profiling of relevant PI cells identified the corticotropin-releasing factor (CRF) homolog, DH44, as a circadian output molecule that is specifically expressed by PI neurons and is required for normal rest:activity rhythms. Notably, selective activation or ablation of just six DH44+ PI cells causes arrhythmicity. These findings delineate a circuit through which clock cells can modulate locomotor rhythms. |
| 巻・号 | 157(3) |
| ページ | 689-701 |
| 公開日 | 2014-4-24 |
| DOI | 10.1016/j.cell.2014.02.024 |
| PII | S0092-8674(14)00223-2 |
| PMID | 24766812 |
| PMC | PMC4003459 |
| MeSH | Animals Animals, Genetically Modified Brain / cytology Brain / physiology Circadian Clocks* Circadian Rhythm Drosophila / cytology Drosophila / physiology* Neurons / cytology Neurons / physiology* Single-Cell Analysis Transcriptome |
| IF | 38.637 |
| 引用数 | 104 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#104906 DGRC#112361 |