| RRC ID |
32515
|
| 著者 |
Cavanaugh DJ, Geratowski JD, Wooltorton JR, Spaethling JM, Hector CE, Zheng X, Johnson EC, Eberwine JH, Sehgal A.
|
| タイトル |
Identification of a circadian output circuit for rest:activity rhythms in Drosophila.
|
| ジャーナル |
Cell
|
| Abstract |
Though much is known about the cellular and molecular components of the circadian clock, output pathways that couple clock cells to overt behaviors have not been identified. We conducted a screen for circadian-relevant neurons in the Drosophila brain and report here that cells of the pars intercerebralis (PI), a functional homolog of the mammalian hypothalamus, comprise an important component of the circadian output pathway for rest:activity rhythms. GFP reconstitution across synaptic partners (GRASP) analysis demonstrates that PI cells are connected to the clock through a polysynaptic circuit extending from pacemaker cells to PI neurons. Molecular profiling of relevant PI cells identified the corticotropin-releasing factor (CRF) homolog, DH44, as a circadian output molecule that is specifically expressed by PI neurons and is required for normal rest:activity rhythms. Notably, selective activation or ablation of just six DH44+ PI cells causes arrhythmicity. These findings delineate a circuit through which clock cells can modulate locomotor rhythms.
|
| 巻・号 |
157(3)
|
| ページ |
689-701
|
| 公開日 |
2014-4-24
|
| DOI |
10.1016/j.cell.2014.02.024
|
| PII |
S0092-8674(14)00223-2
|
| PMID |
24766812
|
| PMC |
PMC4003459
|
| MeSH |
Animals
Animals, Genetically Modified
Brain / cytology
Brain / physiology
Circadian Clocks*
Circadian Rhythm
Drosophila / cytology
Drosophila / physiology*
Neurons / cytology
Neurons / physiology*
Single-Cell Analysis
Transcriptome
|
| IF |
38.637
|
| 引用数 |
104
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
DGRC#104906
DGRC#112361 |
