論文 - 詳細
| RRC ID | 32519 |
|---|---|
| 著者 | Kamimura K, Ueno K, Nakagawa J, Hamada R, Saitoe M, Maeda N. |
| タイトル | Perlecan regulates bidirectional Wnt signaling at the Drosophila neuromuscular junction. |
| ジャーナル | J Cell Biol |
| Abstract |
Heparan sulfate proteoglycans (HSPGs) play pivotal roles in the regulation of Wnt signaling activity in several tissues. At the Drosophila melanogaster neuromuscular junction (NMJ), Wnt/Wingless (Wg) regulates the formation of both pre- and postsynaptic structures; however, the mechanism balancing such bidirectional signaling remains elusive. In this paper, we demonstrate that mutations in the gene of a secreted HSPG, perlecan/trol, resulted in diverse postsynaptic defects and overproduction of synaptic boutons at NMJ. The postsynaptic defects, such as reduction in subsynaptic reticulum (SSR), were rescued by the postsynaptic activation of the Frizzled nuclear import Wg pathway. In contrast, overproduction of synaptic boutons was suppressed by the presynaptic down-regulation of the canonical Wg pathway. We also show that Trol was localized in the SSR and promoted postsynaptic accumulation of extracellular Wg proteins. These results suggest that Trol bidirectionally regulates both pre- and postsynaptic activities of Wg by precisely distributing Wg at the NMJ. |
| 巻・号 | 200(2) |
| ページ | 219-33 |
| 公開日 | 2013-1-21 |
| DOI | 10.1083/jcb.201207036 |
| PII | jcb.201207036 |
| PMID | 23319599 |
| PMC | PMC3549968 |
| MeSH | Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila Proteins / physiology* Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism* Heparan Sulfate Proteoglycans / genetics Heparan Sulfate Proteoglycans / metabolism Heparan Sulfate Proteoglycans / physiology* Larva / growth & development Larva / metabolism Microscopy, Electron, Transmission Mutation Neuromuscular Junction / growth & development Neuromuscular Junction / metabolism* Neuromuscular Junction / ultrastructure Wnt Signaling Pathway* Wnt1 Protein / metabolism |
| IF | 8.811 |
| 引用数 | 38 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#110807 |