RRC ID 32531
Author Yi W, Zhang Y, Tian Y, Guo J, Li Y, Guo A.
Title A subset of cholinergic mushroom body neurons requires Go signaling to regulate sleep in Drosophila.
Journal Sleep
Abstract STUDY OBJECTIVES:Identifying the neurochemistry and neural circuitry of sleep regulation is critical for understanding sleep and various sleep disorders. Fruit flies display sleep-like behavior, sharing essential features with sleep of vertebrate. In the fruit fly's central brain, the mushroom body (MB) has been highlighted as a sleep center; however, its neurochemical nature remains unclear, and whether it promotes sleep or wake is still a topic of controversy.
DESIGN:We used a video recording system to accurately monitor the locomotor activity and sleep status. Gene expression was temporally and regionally manipulated by heat induction and the Gal4/UAS system.
MEASUREMENTS AND RESULTS:We found that expressing pertussis toxin (PTX) in the MB by c309-Gal4 to block Go activity led to unique sleep defects as dramatic sleep increase in daytime and fragmented sleep in nighttime. We narrowed down the c309-Gal4 expressing brain regions to the MB α/β core neurons that are responsible for the Go-mediated sleep effects. Using genetic tools of neurotransmitter-specific Gal80 and RNA interference approach to suppress acetylcholine signal, we demonstrated that these MB α/β core neurons were cholinergic and sleep-promoting neurons, supporting that Go mediates an inhibitory signal. Interestingly, we found that adjacent MB α/β neurons were also cholinergic but wake-promoting neurons, in which Go signal was also required.
CONCLUSION:Our findings in fruit flies characterized a group of sleep-promoting neurons surrounded by a group of wake-promoting neurons. The two groups of neurons are both cholinergic and use Go inhibitory signal to regulate sleep.
Volume 36(12)
Pages 1809-21
Published 2013-12-1
DOI 10.5665/sleep.3206
PMID 24293755
PMC PMC3825430
MeSH Animals Cholinergic Neurons / physiology* Circadian Rhythm / physiology Drosophila melanogaster / physiology Female GTP-Binding Protein alpha Subunits, Gi-Go / physiology* Humans Mushroom Bodies / physiology* Polymerase Chain Reaction Signal Transduction / physiology Sleep / physiology*
IF 4.571
Times Cited 23
WOS Category CLINICAL NEUROLOGY NEUROSCIENCES
Resource
Drosophila sca[109–68]-Gal4 (DGRC#108798) and NP lines