RRC ID 32562
Author Deng W, Casao MC, Wang P, Sato K, Hayes PM, Finnegan EJ, Trevaskis B.
Title Direct links between the vernalization response and other key traits of cereal crops.
Journal Nat Commun
Abstract Transcription of the vernalization1 gene (VRN1) is induced by prolonged cold (vernalization) to trigger flowering of cereal crops, such as wheat and barley. VRN1 encodes a MADS box transcription factor that promotes flowering by regulating the expression of other genes. Here we use transcriptome sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) to identify direct targets of VRN1. Over 500 genomic regions were identified as potential VRN1-binding targets by ChIP-seq. VRN1 binds the promoter of flowering locus T-like 1, a promoter of flowering in vernalized plants. VRN1 also targets vernalization2 and ODDSOC2, repressors of flowering that are downregulated in vernalized plants. RNA-seq identified additional VRN1 targets that might play roles in triggering flowering. Other targets of VRN1 include genes that play central roles in low-temperature-induced freezing tolerance, spike architecture and hormone metabolism. This provides evidence for direct regulatory links between the vernalization response pathway and other important traits in cereal crops.
Volume 6
Pages 5882
Published 2015-1-6
DOI 10.1038/ncomms6882
PII ncomms6882
PMID 25562483
MeSH Acclimatization / genetics Acclimatization / physiology Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Base Sequence Blotting, Western Chromatin Immunoprecipitation Edible Grain / growth & development* Flowers / physiology* Gene Expression Profiling Gene Expression Regulation, Plant / genetics* Gene Expression Regulation, Plant / physiology Hordeum Molecular Sequence Data Plant Growth Regulators / metabolism Plants, Genetically Modified Polymerase Chain Reaction Repressor Proteins / genetics* Repressor Proteins / metabolism Reproduction / physiology Sequence Analysis, RNA Species Specificity
IF 12.353
Times Cited 29
WOS Category PLANT SCIENCES
Resource
Barley barley