| RRC ID |
32562
|
| 著者 |
Deng W, Casao MC, Wang P, Sato K, Hayes PM, Finnegan EJ, Trevaskis B.
|
| タイトル |
Direct links between the vernalization response and other key traits of cereal crops.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Transcription of the vernalization1 gene (VRN1) is induced by prolonged cold (vernalization) to trigger flowering of cereal crops, such as wheat and barley. VRN1 encodes a MADS box transcription factor that promotes flowering by regulating the expression of other genes. Here we use transcriptome sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) to identify direct targets of VRN1. Over 500 genomic regions were identified as potential VRN1-binding targets by ChIP-seq. VRN1 binds the promoter of flowering locus T-like 1, a promoter of flowering in vernalized plants. VRN1 also targets vernalization2 and ODDSOC2, repressors of flowering that are downregulated in vernalized plants. RNA-seq identified additional VRN1 targets that might play roles in triggering flowering. Other targets of VRN1 include genes that play central roles in low-temperature-induced freezing tolerance, spike architecture and hormone metabolism. This provides evidence for direct regulatory links between the vernalization response pathway and other important traits in cereal crops.
|
| 巻・号 |
6
|
| ページ |
5882
|
| 公開日 |
2015-1-6
|
| DOI |
10.1038/ncomms6882
|
| PII |
ncomms6882
|
| PMID |
25562483
|
| MeSH |
Acclimatization / genetics
Acclimatization / physiology
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Base Sequence
Blotting, Western
Chromatin Immunoprecipitation
Edible Grain / growth & development*
Flowers / physiology*
Gene Expression Profiling
Gene Expression Regulation, Plant / genetics*
Gene Expression Regulation, Plant / physiology
Hordeum
Molecular Sequence Data
Plant Growth Regulators / metabolism
Plants, Genetically Modified
Polymerase Chain Reaction
Repressor Proteins / genetics*
Repressor Proteins / metabolism
Reproduction / physiology
Sequence Analysis, RNA
Species Specificity
|
| IF |
12.121
|
| 引用数 |
77
|
|
WOS 分野
|
PLANT SCIENCES
|
| リソース情報 |
| オオムギ |
barley |
