論文 - 詳細
| RRC ID | 32578 |
|---|---|
| 著者 | Yamanaka K, Ishikawa H, Megumi Y, Tokunaga F, Kanie M, Rouault TA, Morishima I, Minato N, Ishimori K, Iwai K. |
| タイトル | Identification of the ubiquitin-protein ligase that recognizes oxidized IRP2. |
| ジャーナル | Nat Cell Biol |
| Abstract |
The ubiquitin system is involved in several basic cellular functions. Ubiquitination is carried out by a cascade of three reactions catalysed by the E1, E2 and E3 enzymes. Among these, the E3 ubiquitin-protein ligases have a pivotal role in determining the specificity of the system by recognizing the target substrates through defined targeting motifs. Although RING finger proteins constitute an important family of E3 ligases, only a few post-transcriptional modifications, including phosphorylation, proline hydroxylation and glycosylation, are known to function as recognition signals for E3. Iron regulatory protein 2 (IRP2), a modulator of iron metabolism, is regulated by iron-induced ubiquitination and degradation. Here we show that the RING finger protein HOIL-1 functions as an E3 ligase for oxidized IRP2, suggesting that oxidation is a specific recognition signal for ubiquitination. The oxidation of IRP2 is generated by haem, which binds to IRP2 in iron-rich cells, and by oxygen, indicating that the iron sensing of IRP2 depends on the synthesis and availability of haem. |
| 巻・号 | 5(4) |
| ページ | 336-40 |
| 公開日 | 2003-4-1 |
| DOI | 10.1038/ncb952 |
| PII | ncb952 |
| PMID | 12629548 |
| MeSH | Amino Acid Sequence / genetics Animals Base Sequence / genetics COS Cells Cloning, Molecular Eukaryotic Cells / enzymology* Gene Expression Regulation, Enzymologic / genetics Genetic Vectors Heme / metabolism Humans Iron / metabolism* Iron Regulatory Protein 2 / genetics Iron Regulatory Protein 2 / metabolism* Ligases / genetics Ligases / isolation & purification* Molecular Sequence Data Mutation / genetics Oxidation-Reduction Transcription Factors Ubiquitin / metabolism* Ubiquitin-Protein Ligases |
| IF | 20.042 |
| 引用数 | 130 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | BS-KS HOIL-1 (RDB04183) |