| RRC ID |
32613
|
| 著者 |
Kurita N, Terao K, Brummer E, Ito E, Nishimura K, Miyaji M.
|
| タイトル |
Resistance of Histoplasma capsulatum to killing by human neutrophils. Evasion of oxidative burst and lysosomal-fusion products.
|
| ジャーナル |
Mycopathologia
|
| Abstract |
The basis for resistance of yeast form of Histoplasma capsulatum to antifungal activity of human neutrophils was studied. In limiting dilution assays and short term coculture assays human neutrophils were ineffective in killing H. capsulatum whereas Candida albicans was readily killed. By contrast, in a cell free hydrogen peroxide-peroxidase-halide system H. capsulatum was as sensitive to killing as C. albicans. Moreover, lysate of human neutrophils effectively substituted for horse-radish peroxidase in a cell free system for killing H. capsulatum. H. capsulatum elicited significant products of the oxidative burst in human neutrophils as detected by luminol-enhanced chemiluminescence. However, the response was two-fold less (p less than 0.05) than that induced by C. albicans. Transmission electron microscopy studies showed that phagosome-lysosome fusion took place when neutrophils phagocytosed C. albicans or H. capsulatum. Taken together, these findings indicate that, even though H. capsulatum elicits an oxidative burst and phagosome-lysosome fusion within the phagosome, it is capable of evading damage in short term assays.
|
| 巻・号 |
115(3)
|
| ページ |
207-13
|
| 公開日 |
1991-9-1
|
| DOI |
10.1007/BF00462229
|
| PMID |
1660960
|
| MeSH |
Cells, Cultured
Histoplasma / immunology*
Histoplasma / ultrastructure
Humans
Hydrogen Peroxide
Lysosomes / physiology*
Microscopy, Electron
Neutrophils / immunology*
Neutrophils / ultrastructure
Peroxidase
Phagocytosis*
Phagosomes / physiology*
Potassium Iodide
Respiratory Burst*
|
| IF |
2.452
|
| 引用数 |
20
|
|
WOS 分野
|
MYCOLOGY
|
| リソース情報 |
| 病原微生物 |
IFM 41329 |
