Reference - Detail
| RRC ID | 3264 |
|---|---|
| Author | Arifuzzaman M, Maeda M, Itoh A, Nishikata K, Takita C, Saito R, Ara T, Nakahigashi K, Huang HC, Hirai A, Tsuzuki K, Nakamura S, Altaf-Ul-Amin M, Oshima T, Baba T, Yamamoto N, Kawamura T, Ioka-Nakamichi T, Kitagawa M, Tomita M, Kanaya S, Wada C, Mori H. |
| Title | Large-scale identification of protein-protein interaction of Escherichia coli K-12. |
| Journal | Genome Res |
| Abstract |
Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested, partners were found for 2667, including 779 of unknown function. Proteins copurifying with hexahistidine-tagged baits on a Ni2+-NTA column were identified by MALDI-TOF MS (matrix-assisted laser desorption ionization time of flight mass spectrometry). An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology. |
| Volume | 16(5) |
| Pages | 686-91 |
| Published | 2006-5-1 |
| DOI | 10.1101/gr.4527806 |
| PII | gr.4527806 |
| PMID | 16606699 |
| PMC | PMC1457052 |
| MeSH | Escherichia coli K12 / chemistry* Escherichia coli Proteins / chemistry Escherichia coli Proteins / metabolism* Gene Library Histidine / chemistry Models, Biological Open Reading Frames Proteome / analysis* Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| IF | 11.093 |
| Times Cited | 300 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY GENETICS & HEREDITY |
| Resource | |
| Prokaryotes E. coli | W3110? |