RRC ID 3271
Author Jovanovic G, Lloyd LJ, Stumpf MP, Mayhew AJ, Buck M.
Title Induction and function of the phage shock protein extracytoplasmic stress response in Escherichia coli.
Journal J. Biol. Chem.
Abstract The phage shock protein (Psp) F regulon response in Escherichia coli is thought to be induced by impaired inner membrane integrity and an associated decrease in proton motive force (pmf). Mechanisms by which the Psp system detects the stress signal and responds have so far remained undetermined. Here we demonstrate that PspA and PspG directly confront a variety of inducing stimuli by switching the cell to anaerobic respiration and fermentation and by down-regulating motility, thereby subtly adjusting and maintaining energy usage and pmf. Additionally, PspG controls iron usage. We show that the Psp-inducing protein IV secretin stress, in the absence of Psp proteins, decreases the pmf in an ArcB-dependent manner and that ArcB is required for amplifying and transducing the stress signal to the PspF regulon. The requirement of the ArcB signal transduction protein for induction of psp provides clear evidence for a direct link between the physiological redox state of the cell, the electron transport chain, and induction of the Psp response. Under normal growth conditions PspA and PspD control the level of activity of ArcB/ArcA system that senses the redox/metabolic state of the cell, whereas under stress conditions PspA, PspD, and PspG deliver their effector functions at least in part by activating ArcB/ArcA through positive feedback.
Volume 281(30)
Pages 21147-61
Published 2006-7-28
DOI 10.1074/jbc.M602323200
PII M602323200
PMID 16709570
MeSH Bacterial Outer Membrane Proteins / metabolism Bacterial Proteins / metabolism* Bacteriophages / metabolism Escherichia coli / metabolism* Escherichia coli Proteins / metabolism* Escherichia coli Proteins / physiology* Gene Expression Regulation, Bacterial Heat-Shock Proteins / metabolism* Membrane Potentials Membrane Proteins / metabolism* Microscopy, Confocal Oxidation-Reduction Plasmids / metabolism Protein Kinases / metabolism Repressor Proteins / metabolism Secretin / metabolism Trans-Activators / metabolism Trans-Activators / physiology*
IF 4.011
Times Cited 71
Prokaryotes E. coli JW5716 JW5536 JW3882 JW2226 JW5536