RRC ID 32717
Author Frankenberg S, Smith L, Greenfield A, Zernicka-Goetz M.
Title Novel gene expression patterns along the proximo-distal axis of the mouse embryo before gastrulation.
Journal BMC Dev Biol
Abstract BACKGROUND:To date, the earliest stage at which the orientation of the anterior-posterior axis in the mouse embryo is distinguishable by asymmetric gene expression is shortly after E5.5. At E5.5, prospective anterior markers are expressed at the distal tip of the embryo, whereas prospective posterior markers are expressed more proximally, close to the boundary with the extraembryonic region.
RESULTS:To contribute to elucidating the mechanisms underlying the events involved in early patterning of the mouse embryo, we have carried out a microarray screen to identify novel genes that are differentially expressed between the distal and proximal parts of the E5.5 embryo. Secondary screening of resulting candidates by in situ hybridisation at E5.5 and E6.5 revealed novel expression patterns for known and previously uncharacterised genes, including Peg10, Ctsz1, Cubilin, Jarid1b, Ndrg1, Sfmbt2, Gjb5, Talia and Plet1. The previously undescribed gene Talia and recently identified Plet1 are expressed specifically in the distal-most part of the extraembryonic ectoderm, adjacent to the epiblast, and are therefore potential candidates for regulating early patterning events. Talia and the previously described gene XE7 define a gene family highly conserved among metazoans and with a predicted protein structure suggestive of a post-transcriptional regulative function, whilst Plet1 appears to be mammal-specific and of unknown function.
CONCLUSION:Our approach has allowed us to compare expression between dissected parts of the egg cylinder and has identified multiple genes with novel expression patterns at this developmental stage. These genes are potential candidates for regulating tissue interactions following implantation.
Volume 7
Pages 8
Published 2007-2-15
DOI 10.1186/1471-213X-7-8
PII 1471-213X-7-8
PMID 17302988
PMC PMC1821012
MeSH Amino Acid Sequence Animals Antigens / genetics Base Sequence Body Patterning / genetics* Embryo, Mammalian / ultrastructure Gastrula / metabolism* Gastrula / ultrastructure Gene Expression Profiling* Membrane Glycoproteins / genetics Mice Molecular Sequence Data Oligonucleotide Array Sequence Analysis RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism Sequence Homology, Amino Acid
IF 2.0
Times Cited 33
WOS Category DEVELOPMENTAL BIOLOGY
Resource
DNA material NIA 15K Mouse cDNA Clone H3001A06 (MCH000406) H3001D07 (MCH000479) H3001E07 (MCH000503) H3004E08 (MCH001704) H3011D11 (MCH004483) H3016G11 (MCH006555) H3017E04 (MCH006900) H3031C12 (MCH012460) H3041C04 (MCH016452).