RRC ID 32797
Author Sasaki K, Ito T, Nishino N, Khochbin S, Yoshida M.
Title Real-time imaging of histone H4 hyperacetylation in living cells.
Journal Proc Natl Acad Sci U S A
Abstract To visualize histone acetylation in living cells, we developed a genetically encoded fluorescent resonance energy transfer (FRET)-based indicator. Response of the indicator reflects changes in the acetylation state of both K5 and K8 in histone H4. Using this acetylation indicator, we were able to monitor the dynamic fluctuation of histone H4 acetylation levels during mitosis, as well as acetylation changes in response to structurally distinct histone deacetylase inhibitors.
Volume 106(38)
Pages 16257-62
Published 2009-9-22
DOI 10.1073/pnas.0902150106
PII 0902150106
PMID 19805290
PMC PMC2752582
MeSH Acetylation / drug effects Animals COS Cells Chlorocebus aethiops Enzyme Inhibitors / pharmacology Fluorescence Resonance Energy Transfer Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HeLa Cells Histone Deacetylase Inhibitors Histone Deacetylases / metabolism Histones / genetics Histones / metabolism* Humans Hydroxamic Acids / pharmacology Immunoblotting Lysine / genetics Lysine / metabolism* Microscopy, Confocal Microscopy, Fluorescence Mitosis Mutation Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism* Time Factors Transfection
IF 9.412
Times Cited 85
DNA material Histac pcDNA3.1 (RDB12840)