Reference - Detail
|Author||Sasaki K, Ito T, Nishino N, Khochbin S, Yoshida M.|
|Title||Real-time imaging of histone H4 hyperacetylation in living cells.|
|Journal||Proc Natl Acad Sci U S A|
To visualize histone acetylation in living cells, we developed a genetically encoded fluorescent resonance energy transfer (FRET)-based indicator. Response of the indicator reflects changes in the acetylation state of both K5 and K8 in histone H4. Using this acetylation indicator, we were able to monitor the dynamic fluctuation of histone H4 acetylation levels during mitosis, as well as acetylation changes in response to structurally distinct histone deacetylase inhibitors.
|MeSH||Acetylation / drug effects Animals COS Cells Chlorocebus aethiops Enzyme Inhibitors / pharmacology Fluorescence Resonance Energy Transfer Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HeLa Cells Histone Deacetylase Inhibitors Histone Deacetylases / metabolism Histones / genetics Histones / metabolism* Humans Hydroxamic Acids / pharmacology Immunoblotting Lysine / genetics Lysine / metabolism* Microscopy, Confocal Microscopy, Fluorescence Mitosis Mutation Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism* Time Factors Transfection|
|WOS Category||BIOCHEMISTRY & MOLECULAR BIOLOGY|
|DNA material||Histac pcDNA3.1 (RDB12840)|